› 8k0a

Electron Microscopy
3.12Å resolution

CryoEM structure of 3-phenylpropionate/cinnamic acid dioxygenase HcaE-HcaF complex

Released:
DOI: 10.2210/pdb8k0a/pdb
PDB entry 8k0a coloured by chain, front view.
PDB entry 8k0a coloured by chain, side view.
PDB entry 8k0a coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Jiang WX, Cheng XQ, Ma LX, Xing Q
Related structures: EMD-36758

Function and Biology Details

Reaction catalysed: 
(2E)-3-phenylprop-2-enoate + NADH + O(2) = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246917 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 453 amino acids
Theoretical weight: 51.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0ABR5 (Residues: 1-453; Coverage: 100%)
Gene names: JW2522, b2538, digA, hcaA, hcaA1, hcaE, phdC1, yfhU
Sequence domains:
3-phenylpropionate/cinnamic acid dioxygenase subunit beta Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 172 amino acids
Theoretical weight: 20.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q47140 (Residues: 1-172; Coverage: 100%)
Gene names: JW2523, b2539, digB, hcaA2, hcaB, hcaF, phdC2, yfhV
Sequence domains: Ring hydroxylating beta subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.12Å
Relevant EMDB volumes: EMD-36758
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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