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Structure of BtKY72 spike receptor-binding domain (RBD) complexed with bat ACE2

Electron Microscopy
3.2Å resolution
PDB entry 8k4u coloured by chain, front view.
PDB entry 8k4u coloured by chain, side view.
PDB entry 8k4u coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Btky72 and Ace2
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Multimeric state: hetero dimer
Accessible surface area: 33690.24 Å2
Buried surface area: 1647.23 Å2
Dissociation area: 773.79 Å2
Dissociation energy (ΔGdiss): -2.24 kcal/mol
Dissociation entropy (TΔSdiss): 12.98 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-246069

Macromolecules

BtKY72
Chain: A
Length: 228 amino acids
Theoretical weight: 25.51 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Homo sapiens
BtKY72 in PDB entry 8k4u, assembly 1, front view.
BtKY72 in PDB entry 8k4u, assembly 1, side view.
BtKY72 in PDB entry 8k4u, assembly 1, top view.
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ACE2
Chain: B
Length: 597 amino acids
Theoretical weight: 69.44 KDa
Source organism: Rhinolophus landeri
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
ACE2 in PDB entry 8k4u, assembly 1, front view.
ACE2 in PDB entry 8k4u, assembly 1, side view.
ACE2 in PDB entry 8k4u, assembly 1, top view.
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