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Crystal structure of NFIL3 in complex with TTACGTAA DNA

X-ray diffraction
2.07Å resolution
PDB entry 8k8a coloured by chain, front view.
PDB entry 8k8a coloured by chain, side view.
PDB entry 8k8a coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nuclear factor interleukin-3-regulated protein and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 12349.28 Å2
Buried surface area: 5432.69 Å2
Dissociation area: 1,296.61 Å2
Dissociation energy (ΔGdiss): 19.39 kcal/mol
Dissociation entropy (TΔSdiss): 10.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-240737

Macromolecules

Nuclear factor interleukin-3-regulated protein
Chains: A, B
Length: 73 amino acids
Theoretical weight: 8.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16649 (Residues: 68-136; Coverage: 15%)
Gene names: E4BP4, IL3BP1, NFIL3
Pfam: Basic region leucine zipper
Nuclear factor interleukin-3-regulated protein in PDB entry 8k8a, assembly 1, front view.
Nuclear factor interleukin-3-regulated protein in PDB entry 8k8a, assembly 1, side view.
Nuclear factor interleukin-3-regulated protein in PDB entry 8k8a, assembly 1, top view.
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DNA chains C, D
Name: DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*G)-3')
Representative chains: C, D
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*G)-3') in PDB entry 8k8a, assembly 1, front view.
DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*G)-3') in PDB entry 8k8a, assembly 1, side view.
DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*G)-3') in PDB entry 8k8a, assembly 1, top view.
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