› 8k8d

X-ray diffraction
2.2Å resolution

Crystal structure of C/EBPbeta BZIP domain bound to a high affinity DNA

Released:
DOI: 10.2210/pdb8k8d/pdb
PDB entry 8k8d coloured by chain, front view.
PDB entry 8k8d coloured by chain, side view.
PDB entry 8k8d coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for specific DNA sequence recognition by the transcription factor NFIL3.
Chen S, Lei M, Liu K, Min J
J Biol Chem 105776 (2024)
PMID: 38382670

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240739 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
CCAAT/enhancer-binding protein beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 79 amino acids
Theoretical weight: 9.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 259-336; Coverage: 23%)
Gene names: CEBPB, PP9092, TCF5
Sequence domains: Basic region leucine zipper
DNA (5'-D(*CP*AP*TP*TP*AP*CP*GP*TP*AP*AP*TP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 63.668Å b: 46.4Å c: 98.783Å
α: 90° β: 108.76° γ: 90°
R-values:
R R work R free
0.239 0.236 0.292
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links