8kai

X-ray diffraction
3.49Å resolution

Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 17nt target DNA

Released:
Model geometry
Fit model/data

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-270588 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
DNA (25-MER) Chains: C, G
Molecule details ›
Chains: C, G
Length: 25 nucleotides
Theoretical weight: 7.57 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3') Chains: D, H
RNA (34-MER) Chains: A, E
RNA (65-MER) Chains: I, J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 144.3Å b: 130.186Å c: 146.413Å
α: 90° β: 104.021° γ: 90°
R-values:
R R work R free
0.228 0.225 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Escherichia coli DH5[alpha]