› 8kda

Electron Microscopy
3.19Å resolution

Cryo-EM structure of Hydrogenobacter thermophilus minimal protein-only RNase P (HARP) in complex with pre-tRNAs

Released:
DOI: 10.2210/pdb8kda/pdb
PDB entry 8kda coloured by chain, front view.
PDB entry 8kda coloured by chain, side view.
PDB entry 8kda coloured by chain, top view.
Source organisms:
Entry authors: Teramoto T, Adachi N, Yokogawa T, Koyasu T, Mayanagi K, Nakamura T, Senda T, Kakuta Y
Related structures: EMD-37129

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-249579 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-free ribonuclease P Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 189 amino acids
Theoretical weight: 21.94 KDa
Source organism: Hydrogenobacter thermophilus TK-6
Expression system: Escherichia coli
UniProt:
  • Canonical: D3DIV8 (Residues: 1-189; Coverage: 97%)
Gene name: HTH_1307
Sequence domains: PINc domain ribonuclease
Aquifex aeolicus pre-tRNAVal Chains: M, N, O, P, T
Molecule details ›
Chains: M, N, O, P, T
Length: 73 nucleotides
Theoretical weight: 23.65 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-37129
Expression systems:
  • Escherichia coli
  • Not provided

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