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Cryo-EM structure of Hydrogenobacter thermophilus minimal protein-only RNase P (HARP) in complex with pre-tRNAs

Electron Microscopy
3.19Å resolution
PDB entry 8kda coloured by chain, front view.
PDB entry 8kda coloured by chain, side view.
PDB entry 8kda coloured by chain, top view.
Source organisms:
Assembly composition:
hetero heptadecamer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: RNA-free ribonuclease P and RNA
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Multimeric state: hetero heptadecamer
Accessible surface area: 143773.93 Å2
Buried surface area: 51754.97 Å2
Dissociation area: 10,283.67 Å2
Dissociation energy (ΔGdiss): 70.1 kcal/mol
Dissociation entropy (TΔSdiss): 77.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-249579

Macromolecules

RNA-free ribonuclease P
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 189 amino acids
Theoretical weight: 21.94 KDa
Source organism: Hydrogenobacter thermophilus TK-6
Expression system: Escherichia coli
UniProt:
  • Canonical: D3DIV8 (Residues: 1-189; Coverage: 97%)
Gene name: HTH_1307
Pfam: PINc domain ribonuclease
RNA-free ribonuclease P in PDB entry 8kda, assembly 1, front view.
RNA-free ribonuclease P in PDB entry 8kda, assembly 1, side view.
RNA-free ribonuclease P in PDB entry 8kda, assembly 1, top view.
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RNA chains M, N, O, P, T
Name: Aquifex aeolicus pre-tRNAVal
Representative chains: M, N, O, P, T
Source organism: Aquifex aeolicus [63363]
Expression system: Not provided
Length: 73 nucleotides
Theoretical weight: 23.65 KDa
Rfam: tRNA
Aquifex aeolicus pre-tRNAVal in PDB entry 8kda, assembly 1, front view.
Aquifex aeolicus pre-tRNAVal in PDB entry 8kda, assembly 1, side view.
Aquifex aeolicus pre-tRNAVal in PDB entry 8kda, assembly 1, top view.
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