8kh5

Electron Microscopy
2.83Å resolution

Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-232468 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Probable G-protein coupled receptor 174 Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 39.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BXC1 (Residues: 2-328; Coverage: 98%)
Gene names: FKSG79, GPCR17, GPR174
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: B
Molecule details ›
Chain: B
Length: 248 amino acids
Theoretical weight: 28.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 6-64, 204-394; Coverage: 61%)
  • Best match: P63092-3 (Residues: 6-64, 189-379)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Nanobody 35 Chain: E
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.83Å
Relevant EMDB volumes: EMD-37237
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)