› 8kh5

Electron Microscopy
2.83Å resolution

Cryo-EM structure of the GPR174-Gs complex bound to endogenous lysoPS

Released:
DOI: 10.2210/pdb8kh5/pdb
PDB entry 8kh5 coloured by chain, front view.
PDB entry 8kh5 coloured by chain, side view.
PDB entry 8kh5 coloured by chain, top view.
Source organisms:
Primary publication:
Specific binding of GPR174 by endogenous lysophosphatidylserine leads to high constitutive Gs signaling.
Nie Y, Qiu Z, Chen S, Chen Z, Song X, Ma Y, Huang N, Cyster JG, Zheng S
OpenAccess logo Nat Commun 14 5901 (2023)
PMID: 37737235
Related structures: EMD-37237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-232468 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Probable G-protein coupled receptor 174 Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 39.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BXC1 (Residues: 2-328; Coverage: 98%)
Gene names: FKSG79, GPCR17, GPR174
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: B
Molecule details ›
Chain: B
Length: 248 amino acids
Theoretical weight: 28.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 6-64, 204-394; Coverage: 61%)
  • Best match: P63092-3 (Residues: 6-64, 189-379)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 357 amino acids
Theoretical weight: 39.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Nanobody 35 Chain: E
Molecule details ›
Chain: E
Length: 160 amino acids
Theoretical weight: 17.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

2 bound ligands:
Ligand CLR 2 x CLR
Ligand LPS 1 x LPS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.83Å
Relevant EMDB volumes: EMD-37237
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway.
Hoppe et al. (2024)
2 more