8oiv

X-ray diffraction
2.12Å resolution

Monkeypox virus VP39 in complex with SAH and cap0

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-498602 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 35.95 KDa
Source organism: Monkeypox virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A7H0DN74 (Residues: 1-307; Coverage: 92%)
Gene names: MPXV085, OPG102, PAPS
Sequence domains: Poly A polymerase regulatory subunit
mGpppGAAAAA Chain: D
Molecule details ›
Chain: D
Length: 6 nucleotides
Theoretical weight: 2.39 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 52.77Å b: 84.89Å c: 155.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided