Structure analysis

60S ribosomal subunit bound to the E3-UFM1 complex - state 2 (native)

Electron Microscopy
3.3Å resolution
Assembly composition:
hetero 53-mer (preferred)
Entry contents: 50 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 53-mer
Accessible surface area: 753909.54 Å2
Buried surface area: 446049.09 Å2
Dissociation area: 1,402.68 Å2
Dissociation energy (ΔGdiss): 3.87 kcal/mol
Dissociation entropy (TΔSdiss): 16.08 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-240072

Macromolecules

Chain: 1
Length: 476 amino acids
Theoretical weight: 52.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P38377 (Residues: 1-476; Coverage: 100%)
Gene name: SEC61A1
Pfam:

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Chain: 2
Length: 68 amino acids
Theoretical weight: 7.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60059 (Residues: 1-68; Coverage: 100%)
Gene name: SEC61G
Pfam: SecE/Sec61-gamma subunits of protein translocation complex

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Chain: 3
Length: 96 amino acids
Theoretical weight: 9.99 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60468 (Residues: 1-96; Coverage: 100%)
Gene name: SEC61B
Pfam: Sec61beta family

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Chain: A
Length: 794 amino acids
Theoretical weight: 89.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O94874 (Residues: 1-794; Coverage: 100%)
Gene names: KIAA0776, MAXER, NLBP, RCAD, UFL1
Pfam: E3 UFM1-protein ligase 1

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Chain: B
Length: 506 amino acids
Theoretical weight: 56.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96JB5 (Residues: 1-506; Coverage: 100%)
Gene names: CDK5RAP3, IC53, LZAP, MSTP016, OK/SW-cl.114, PP1553
Pfam: CDK5 regulatory subunit-associated protein 3

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Chain: C
Length: 314 amino acids
Theoretical weight: 35.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96HY6 (Residues: 1-314; Coverage: 100%)
Gene names: C20orf116, DDRGK1, UFBP1
Pfam: DDRGK domain

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Chain: D
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61960 (Residues: 1-85; Coverage: 100%)
Gene names: BM-002, C13orf20, UFM1
Pfam: Ubiquitin fold modifier 1 protein

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Chain: K
Length: 245 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P56537 (Residues: 1-245; Coverage: 100%)
Gene names: EIF3A, EIF6, ITGB4BP, OK/SW-cl.27
Pfam: eIF-6 family

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Chain: LA
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62917 (Residues: 1-257; Coverage: 100%)
Gene name: RPL8
Pfam:

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Chain: LB
Length: 403 amino acids
Theoretical weight: 46.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P39023 (Residues: 1-403; Coverage: 100%)
Gene names: OK/SW-cl.32, RPL3
Pfam: Ribosomal protein L3

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Chain: LC
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P36578 (Residues: 1-427; Coverage: 100%)
Gene names: RPL1, RPL4
Pfam:

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Chain: LD
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46777 (Residues: 1-297; Coverage: 100%)
Gene names: MSTP030, RPL5
Pfam:

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Chain: LE
Length: 288 amino acids
Theoretical weight: 32.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02878 (Residues: 1-288; Coverage: 100%)
Gene names: RPL6, TXREB1
Pfam:

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Chain: LF
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18124 (Residues: 1-248; Coverage: 100%)
Gene name: RPL7
Pfam:

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Chain: LG
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62424 (Residues: 1-266; Coverage: 100%)
Gene names: RPL7A, SURF-3, SURF3
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: LH
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P32969 (Residues: 1-192; Coverage: 100%)
Gene names: OK/SW-cl.103, RPL9, RPL9P7, RPL9P8, RPL9P9
Pfam: Ribosomal protein L6

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Chain: LI
Length: 214 amino acids
Theoretical weight: 24.55 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96L21 (Residues: 1-214; Coverage: 100%)
Gene name: RPL10L
Pfam: Ribosomal protein L16p/L10e

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Chain: LJ
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62913 (Residues: 1-178; Coverage: 100%)
Gene name: RPL11
Pfam:

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Chain: LL
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P26373 (Residues: 1-211; Coverage: 100%)
Gene names: BBC1, OK/SW-cl.46, RPL13
Pfam: Ribosomal protein L13e

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Chain: LM
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P50914 (Residues: 1-215; Coverage: 100%)
Gene name: RPL14
Pfam: Ribosomal protein L14

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Chain: LN
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61313 (Residues: 1-204; Coverage: 100%)
Gene names: EC45, RPL15, TCBAP0781
Pfam: Ribosomal L15

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Chain: LO
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40429 (Residues: 1-203; Coverage: 100%)
Gene name: RPL13A
Pfam: Ribosomal protein L13

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Chain: LP
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18621 (Residues: 1-184; Coverage: 100%)
Gene name: RPL17
Pfam: Ribosomal protein L22p/L17e

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Chain: LQ
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q07020 (Residues: 1-188; Coverage: 100%)
Gene name: RPL18
Pfam: Ribosomal protein 60S L18 and 50S L18e

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Chain: LR
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P84098 (Residues: 1-196; Coverage: 100%)
Gene name: RPL19
Pfam: Ribosomal protein L19e

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Chain: LS
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02543 (Residues: 1-176; Coverage: 100%)
Gene name: RPL18A
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

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Chain: LT
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46778 (Residues: 1-160; Coverage: 100%)
Gene name: RPL21
Pfam: Ribosomal protein L21e

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Chain: LU
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35268 (Residues: 1-128; Coverage: 100%)
Gene name: RPL22
Pfam: Ribosomal L22e protein family

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Chain: LV
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62829 (Residues: 1-140; Coverage: 100%)
Gene name: RPL23
Pfam: Ribosomal protein L14p/L23e

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Chain: LW
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P83731 (Residues: 1-157; Coverage: 100%)
Gene name: RPL24
Pfam: Ribosomal protein L24e

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Chain: LX
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62750 (Residues: 1-156; Coverage: 100%)
Gene name: RPL23A
Pfam:

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Chain: LY
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61254 (Residues: 1-145; Coverage: 100%)
Gene name: RPL26
Pfam:

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Chain: LZ
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61353 (Residues: 1-136; Coverage: 100%)
Gene name: RPL27
Pfam:

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Chain: La
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46776 (Residues: 1-148; Coverage: 100%)
Gene name: RPL27A
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: Lb
Length: 159 amino acids
Theoretical weight: 17.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47914 (Residues: 1-159; Coverage: 100%)
Gene name: RPL29
Pfam: Ribosomal L29e protein family

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Chain: Lc
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62888 (Residues: 1-115; Coverage: 100%)
Gene name: RPL30
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: Ld
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62899 (Residues: 1-125; Coverage: 100%)
Gene name: RPL31
Pfam: Ribosomal protein L31e

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Chain: Le
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62910 (Residues: 1-135; Coverage: 100%)
Gene names: PP9932, RPL32
Pfam: Ribosomal protein L32

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Chain: Lf
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18077 (Residues: 1-110; Coverage: 100%)
Gene names: GIG33, RPL35A
Pfam: Ribosomal protein L35Ae

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Chain: Lg
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens environmental sample
UniProt:
  • Canonical: A0A6J3FY29 (Residues: 1-117; Coverage: 100%)
Gene name: RPL34
Pfam: Ribosomal protein L34e

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Chain: Lh
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42766 (Residues: 1-123; Coverage: 100%)
Gene name: RPL35
Pfam: Ribosomal L29 protein

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Chain: Li
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y3U8 (Residues: 1-105; Coverage: 100%)
Gene name: RPL36
Pfam: Ribosomal protein L36e

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Chain: Lj
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61927 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Pfam: Ribosomal protein L37e

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Chain: Lk
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63173 (Residues: 1-70; Coverage: 100%)
Gene name: RPL38
Pfam: Ribosomal L38e protein family

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Chain: Ll
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62891 (Residues: 1-51; Coverage: 100%)
Gene name: RPL39
Pfam: Ribosomal L39 protein

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Chain: Lm
Length: 128 amino acids
Theoretical weight: 14.76 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62987 (Residues: 1-128; Coverage: 100%)
Gene names: UBA52, UBCEP2
Pfam:

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Chain: Lo
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P83881 (Residues: 1-106; Coverage: 100%)
Gene names: GIG15, MIG6, RPL36A, RPL44
Pfam: Ribosomal protein L44

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Chain: Lp
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61513 (Residues: 1-92; Coverage: 100%)
Gene name: RPL37A
Pfam: Ribosomal L37ae protein family

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Chain: Lr
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46779 (Residues: 1-137; Coverage: 100%)
Gene name: RPL28
Pfam: Ribosomal L28e protein family

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Chain: Lz
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62906 (Residues: 1-217; Coverage: 100%)
Gene names: NEDD6, RPL10A
Pfam: Ribosomal protein L1p/L10e family

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Name: 28S ribosomal RNA
Representative chains: 5
Length: 5070 nucleotides
Theoretical weight: 1640.17 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: 7
Length: 121 nucleotides
Theoretical weight: 39 KDa
Rfam: 5S ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: 8
Length: 157 nucleotides
Theoretical weight: 50.45 KDa
Rfam: 5.8S ribosomal RNA

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