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Human pseudouridine synthase 3 and tRNA-Gln

Electron Microscopy
3.1Å resolution
PDB entry 8okd coloured by chain, front view.
PDB entry 8okd coloured by chain, side view.
PDB entry 8okd coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: tRNA pseudouridine(38/39) synthase and RNA
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Multimeric state: hetero tetramer
Accessible surface area: 49000.71 Å2
Buried surface area: 10279.45 Å2
Dissociation area: 3,132.76 Å2
Dissociation energy (ΔGdiss): 33.28 kcal/mol
Dissociation entropy (TΔSdiss): 27.79 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-247011

Macromolecules

tRNA pseudouridine(38/39) synthase
Chains: A, B
Length: 482 amino acids
Theoretical weight: 55.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BZE2 (Residues: 4-481; Coverage: 99%)
Gene names: FKSG32, PUS3
Pfam: tRNA pseudouridine synthase
tRNA pseudouridine(38/39) synthase in PDB entry 8okd, assembly 1, front view.
tRNA pseudouridine(38/39) synthase in PDB entry 8okd, assembly 1, side view.
tRNA pseudouridine(38/39) synthase in PDB entry 8okd, assembly 1, top view.
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RNA chains C, X
Name: human tRNA-Gln (70-MER)
Representative chains: C, X
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 75 nucleotides
Theoretical weight: 24.05 KDa
human tRNA-Gln (70-MER) in PDB entry 8okd, assembly 1, front view.
human tRNA-Gln (70-MER) in PDB entry 8okd, assembly 1, side view.
human tRNA-Gln (70-MER) in PDB entry 8okd, assembly 1, top view.
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