› 8opx

X-ray diffraction
2.9Å resolution

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129474 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Probable fatty oxidation protein FadB Chains: A, B
Molecule details ›
Chains: A, B
Length: 736 amino acids
Theoretical weight: 78.01 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53872 (Residues: 1-720; Coverage: 100%)
Gene names: Rv0860, fadB
Sequence domains:
Putative acyltransferase Rv0859 Chains: C, D
Molecule details ›
Chains: C, D
Length: 403 amino acids
Theoretical weight: 42.46 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53871 (Residues: 1-403; Coverage: 100%)
Gene names: Rv0859, fadA
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 24 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 248.41Å b: 135.164Å c: 119.158Å
α: 90° β: 110.565° γ: 90°
R-values:
R R work R free
0.2 0.198 0.229
Expression system: Escherichia coli BL21(DE3)

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