› 8p4o

Electron Microscopy
3.04Å resolution

CryoEM structure of a GroEL7-GroES7 cage with encapsulated ordered substrate MetK in the presence of ADP-BeFx

Released:
DOI: 10.2210/pdb8p4o/pdb
PDB entry 8p4o coloured by chain, front view.
PDB entry 8p4o coloured by chain, side view.
PDB entry 8p4o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Visualizing chaperonin function in situ by cryo-electron tomography
Wagner J, Caravajal AI, Beck F, Bracher A, Wan W, Bohn S, Koerner R, Baumeister W, Fernandez-Busnadiego R, Hartl FU
Nature (2024)
Related structures: EMD-17421

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-246600 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chaperonin GroEL Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 547 amino acids
Theoretical weight: 57.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F5 (Residues: 2-548; Coverage: 100%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Co-chaperonin GroES Chains: O, P, Q, R, S, T, U
Molecule details ›
Chains: O, P, Q, R, S, T, U
Length: 97 amino acids
Theoretical weight: 10.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F9 (Residues: 1-97; Coverage: 100%)
Gene names: JW4102, b4142, groES, groS, mopB
Sequence domains: Chaperonin 10 Kd subunit
S-adenosylmethionine synthase Chain: 1
Molecule details ›
Chain: 1
Length: 384 amino acids
Theoretical weight: 42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A817 (Residues: 1-384; Coverage: 100%)
Gene names: JW2909, b2942, metK, metX
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 7 x ADP
Ligand MG 7 x MG
Ligand BEF 7 x BEF
Ligand K 7 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.04Å
Relevant EMDB volumes: EMD-17421
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21

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