› 8p8j

Electron Microscopy
3.49Å resolution

Structure of 5D3-Fab and nanobody(Nb96)-bound ABCG2

Released:
DOI: 10.2210/pdb8p8j/pdb
PDB entry 8p8j coloured by chain, front view.
PDB entry 8p8j coloured by chain, side view.
PDB entry 8p8j coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the allosteric inhibition of human ABCG2 by nanobodies.
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol 168234 (2023)
PMID: 37597690
Related structures: EMD-17547

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227952 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Broad substrate specificity ATP-binding cassette transporter ABCG2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 72.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UNQ0 (Residues: 1-655; Coverage: 100%)
Gene names: ABCG2, ABCP, BCRP, BCRP1, MXR
Sequence domains:
5D3(Fab) light chain variable domain Chains: C, E
Molecule details ›
Chains: C, E
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
Expression system: Mus musculus
5D3(Fab) heavy chain variable domain Chains: D, F
Molecule details ›
Chains: D, F
Length: 221 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Nanobody Chain: Z
Molecule details ›
Chain: Z
Length: 125 amino acids
Theoretical weight: 13.7 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.49Å
Relevant EMDB volumes: EMD-17547
Expression systems:
  • Homo sapiens
  • Mus musculus
  • Escherichia coli

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