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Crystal Structure of a Squalene-Hopene Cyclase from Cystobacter fuscus

X-ray diffraction
1.42Å resolution
PDB entry 8pak coloured by chain, front view.
PDB entry 8pak coloured by chain, side view.
PDB entry 8pak coloured by chain, top view.
Source organism: Cystobacter fuscus DSM 2262
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Squalene cyclase C-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 14385.9 Å2
Buried surface area: 607.77 Å2
Dissociation area: 113.42 Å2
Dissociation energy (ΔGdiss): -2.25 kcal/mol
Dissociation entropy (TΔSdiss): -0.09 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-246174
Assembly 2 (preferred)
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Assembly Name: Squalene cyclase C-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 14095.28 Å2
Buried surface area: 1647.58 Å2
Dissociation area: 100.11 Å2
Dissociation energy (ΔGdiss): -3.05 kcal/mol
Dissociation entropy (TΔSdiss): -0.11 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-246174

Macromolecules

Squalene cyclase C-terminal domain-containing protein
Chains: A, B
Length: 361 amino acids
Theoretical weight: 39.65 KDa
Source organism: Cystobacter fuscus DSM 2262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A250J6H4 (Residues: 1-361; Coverage: 100%)
Gene name: CYFUS_004654
Pfam:
Squalene cyclase C-terminal domain-containing protein in PDB entry 8pak, assembly 2, front view.
Squalene cyclase C-terminal domain-containing protein in PDB entry 8pak, assembly 2, side view.
Squalene cyclase C-terminal domain-containing protein in PDB entry 8pak, assembly 2, top view.
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