› 8pem

X-ray diffraction
2Å resolution

Zika Methyltransferase in complex with AT-9010 and SAH

Released:
DOI: 10.2210/pdb8pem/pdb
PDB entry 8pem coloured by chain, front view.
PDB entry 8pem coloured by chain, side view.
PDB entry 8pem coloured by chain, top view.
Source organism: Zika virus
Entry authors: Krejcova K, Boura E, Klima M

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase NS5 Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.32 KDa
Source organism: Zika virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q32ZE1 (Residues: 2517-2780; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
3 bound ligands:
Ligand AT9 1 x AT9
Ligand SO4 1 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 39.556Å b: 41.016Å c: 69.035Å
α: 90° β: 96.25° γ: 90°
R-values:
R R work R free
0.23 0.228 0.262
Expression system: Escherichia coli BL21(DE3)

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