› 8pm2

Electron Microscopy
2.92Å resolution

Structure of the murine trace amine-associated receptor TAAR7f bound to N,N-dimethylcyclohexylamine (DMCH) in complex with mini-Gs trimeric G protein

Released:
DOI: 10.2210/pdb8pm2/pdb
PDB entry 8pm2 coloured by chain, front view.
PDB entry 8pm2 coloured by chain, side view.
PDB entry 8pm2 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular recognition of an aversive odorant by the murine trace amine-associated receptor TAAR7f.
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
OpenAccess logo bioRxiv (2023)
PMID: 37461561
Related structures: EMD-17756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-239132 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63092 (Residues: 5-394; Coverage: 63%)
  • Best match: P63092-2 (Residues: 5-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.34 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 156 amino acids
Theoretical weight: 16.93 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21
Trace amine-associated receptor 7f Chain: R
Molecule details ›
Chain: R
Length: 365 amino acids
Theoretical weight: 41.01 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5QD08 (Residues: 1-358; Coverage: 100%)
Gene name: Taar7f
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

2 bound ligands:
Ligand Y01 1 x Y01
Ligand 8IA 1 x 8IA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.92Å
Relevant EMDB volumes: EMD-17756
Expression systems:
  • Escherichia coli BL21
  • Trichoplusia ni

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