› 8prw

Electron Microscopy
1.9Å resolution

Cryo-EM structure of the yeast fatty acid synthase at 1.9 angstrom resolution

Released:
DOI: 10.2210/pdb8prw/pdb
PDB entry 8prw coloured by chain, front view.
PDB entry 8prw coloured by chain, side view.
PDB entry 8prw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots.
Singh K, Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
Cell 186 5054-5067.e16 (2023)
PMID: 37949058
Related structures: EMD-17840

Function and Biology Details

Reactions catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+)
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139405 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fatty acid synthase subunit alpha Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 1887 amino acids
Theoretical weight: 207.18 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P19097 (Residues: 1-1887; Coverage: 100%)
Gene names: FAS2, P1409, YPL231W
Sequence domains:
Fatty acid synthase subunit beta Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 2051 amino acids
Theoretical weight: 229.03 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07149 (Residues: 1-2051; Coverage: 100%)
Gene names: FAS1, YKL182W
Sequence domains:

Ligands and Environments


Cofactor: Ligand FNR 6 x FNR

Cofactor: Ligand COA 6 x COA

Cofactor: Ligand NAP 12 x NAP
No bound ligands
1 modified residue:
Ligand J8W 6 x J8W

Experiments and Validation Details

Entry percentile scores
Resolution: 1.9Å
Relevant EMDB volumes: EMD-17840

Quick links

Citations

1 citations in other articles

Direct structural analysis of a single acyl carrier protein domain in fatty acid synthase from the fungus Saccharomyces cerevisiae.
Samani et al. (2024)