› 8pt2

Electron Microscopy
2.59Å resolution

Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (transcriptase conformation)

Released:
DOI: 10.2210/pdb8pt2/pdb
PDB entry 8pt2 coloured by chain, front view.
PDB entry 8pt2 coloured by chain, side view.
PDB entry 8pt2 coloured by chain, top view.
Source organism: Tilapia lake virus
Primary publication:
Structural and functional analysis of the minimal orthomyxovirus-like polymerase of Tilapia Lake Virus from the highly diverged Amnoonviridae family.
Arragain B, Pelosse M, Thompson A, Cusack S
OpenAccess logo Nat Commun 14 8145 (2023)
PMID: 38066000
Related structures: EMD-17866

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-237494 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Polymerase acidic protein (PA-like) Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 47.78 KDa
Source organism: Tilapia lake virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A142I7Z3 (Residues: 1-419; Coverage: 100%)
Putative PB1 Chain: B
Molecule details ›
Chain: B
Length: 519 amino acids
Theoretical weight: 57.18 KDa
Source organism: Tilapia lake virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A1Y9SHW4 (Residues: 1-519; Coverage: 100%)
Gene name: PB1
RNA-dependent RNA polymerase Chain: C
Molecule details ›
Chain: C
Length: 478 amino acids
Theoretical weight: 53.78 KDa
Source organism: Tilapia lake virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A7G3S745 (Residues: 1-457; Coverage: 100%)
5' vRNA end - vRNA loop (40-mer) Chains: D, V
Molecule details ›
Chains: D, V
Length: 40 nucleotides
Theoretical weight: 12.74 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.59Å
Relevant EMDB volumes: EMD-17866
Expression system: Trichoplusia ni

Quick links

Citations

1 citations in other articles

ICTV Virus Taxonomy Profile: Amnoonviridae 2023.
Koonin et al. (2023)

1 mention without citation

Structural and functional analysis of the minimal orthomyxovirus-like polymerase of Tilapia Lake Virus from the highly diverged Amnoonviridae family.
Arragain et al. (2023)