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Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (transcriptase conformation)

Electron Microscopy
2.59Å resolution
PDB entry 8pt2 coloured by chain, front view.
PDB entry 8pt2 coloured by chain, side view.
PDB entry 8pt2 coloured by chain, top view.
Source organism: Tilapia lake virus
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 59638.63 Å2
Buried surface area: 26876.87 Å2
Dissociation area: 1,806.34 Å2
Dissociation energy (ΔGdiss): 4.64 kcal/mol
Dissociation entropy (TΔSdiss): 11.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-237494

Macromolecules

Polymerase acidic protein (PA-like)
Chain: A
Length: 419 amino acids
Theoretical weight: 47.78 KDa
Source organism: Tilapia lake virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A142I7Z3 (Residues: 1-419; Coverage: 100%)
Polymerase acidic protein (PA-like) in PDB entry 8pt2, assembly 1, front view.
Polymerase acidic protein (PA-like) in PDB entry 8pt2, assembly 1, side view.
Polymerase acidic protein (PA-like) in PDB entry 8pt2, assembly 1, top view.
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Putative PB1
Chain: B
Length: 519 amino acids
Theoretical weight: 57.18 KDa
Source organism: Tilapia lake virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A1Y9SHW4 (Residues: 1-519; Coverage: 100%)
Gene name: PB1
Putative PB1 in PDB entry 8pt2, assembly 1, front view.
Putative PB1 in PDB entry 8pt2, assembly 1, side view.
Putative PB1 in PDB entry 8pt2, assembly 1, top view.
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RNA-dependent RNA polymerase
Chain: C
Length: 478 amino acids
Theoretical weight: 53.78 KDa
Source organism: Tilapia lake virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A7G3S745 (Residues: 1-457; Coverage: 100%)
RNA-dependent RNA polymerase in PDB entry 8pt2, assembly 1, front view.
RNA-dependent RNA polymerase in PDB entry 8pt2, assembly 1, side view.
RNA-dependent RNA polymerase in PDB entry 8pt2, assembly 1, top view.
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RNA chains D, V
Name: 5' vRNA end - vRNA loop (40-mer)
Representative chains: D, V
Length: 40 nucleotides
Theoretical weight: 12.74 KDa
5' vRNA end - vRNA loop (40-mer) in PDB entry 8pt2, assembly 1, front view.
5' vRNA end - vRNA loop (40-mer) in PDB entry 8pt2, assembly 1, side view.
5' vRNA end - vRNA loop (40-mer) in PDB entry 8pt2, assembly 1, top view.
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