Structure analysis

Chaetomium thermophilum pre-60S State 5 - pre-5S rotation - L1 inward - composite structure

Electron Microscopy
2.55Å resolution
Assembly composition:
hetero 60-mer (preferred)
Entry contents: 55 distinct polypeptide molecules
4 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Assembly Name: 60S ribosome subunit and C2H2-type domain-containing protein and CP-type G domain-containing protein and DUF2423 domain-containing protein and Eukaryo...
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Multimeric state: hetero 60-mer
Accessible surface area: 798929.0 Å2
Buried surface area: 535851.49 Å2
Dissociation area: 3,090.14 Å2
Dissociation energy (ΔGdiss): 17.32 kcal/mol
Dissociation entropy (TΔSdiss): 16.2 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-236226

Macromolecules

Chain: CB
Length: 391 amino acids
Theoretical weight: 43.7 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S7X0 (Residues: 1-391; Coverage: 100%)
Gene name: CTHT_0037280
Pfam: Ribosomal protein L1p/L10e family

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Chain: CF
Length: 270 amino acids
Theoretical weight: 30.53 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S616 (Residues: 1-270; Coverage: 100%)
Gene name: CTHT_0025680
Pfam:

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Chain: CH
Length: 661 amino acids
Theoretical weight: 75.62 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S8F1 (Residues: 1-661; Coverage: 100%)
Gene names: CTHT_0029660, NOG1
Pfam:

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Chain: CI
Length: 414 amino acids
Theoretical weight: 45.88 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S1H0 (Residues: 1-414; Coverage: 100%)
Gene name: CTHT_0013570
Pfam: RNA recognition motif

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Chain: CJ
Length: 679 amino acids
Theoretical weight: 77.17 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SHX6 (Residues: 1-679; Coverage: 100%)
Gene names: CTHT_0073720, NOP7
Pfam:

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Chain: CK
Length: 261 amino acids
Theoretical weight: 29.72 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S081 (Residues: 1-261; Coverage: 100%)
Gene name: CTHT_0009080
Pfam: Ribosomal protein S8e

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Chain: CL
Length: 558 amino acids
Theoretical weight: 61.48 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SEW3 (Residues: 1-558; Coverage: 100%)
Gene name: CTHT_0059920
Pfam:

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Chains: CM, LF
Length: 249 amino acids
Theoretical weight: 28.82 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SFL0 (Residues: 1-249; Coverage: 100%)
Gene name: CTHT_0071220
Pfam:

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Chain: CN
Length: 246 amino acids
Theoretical weight: 26.47 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S683 (Residues: 1-246; Coverage: 100%)
Gene names: CTHT_0025830, TIF6
Pfam: eIF-6 family

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Chain: CO
Length: 120 amino acids
Theoretical weight: 13.26 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SHK0 (Residues: 1-120; Coverage: 100%)
Gene name: CTHT_0070290
Pfam: YBL028C ribosome biogenesis factor, N-terminal domain

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Chain: CQ
Length: 225 amino acids
Theoretical weight: 26.43 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S497 (Residues: 1-225; Coverage: 100%)
Gene name: CTHT_0039400
Pfam: Ribosomal protein L24e

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Chain: Lq
Length: 147 amino acids
Theoretical weight: 15.96 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SHJ6 (Residues: 1-147; Coverage: 100%)
Gene name: CTHT_0070250
Pfam: Ribosomal L28e protein family

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Chain: Cb
Length: 117 amino acids
Theoretical weight: 13.71 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SB32 (Residues: 1-117; Coverage: 100%)
Gene name: CTHT_0048710
Pfam: Zinc-finger double-stranded RNA-binding

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Chain: Cd
Length: 627 amino acids
Theoretical weight: 70.31 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SBX1 (Residues: 1-627; Coverage: 100%)
Gene name: CTHT_0055100
Pfam:

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Chain: Ce
Length: 443 amino acids
Theoretical weight: 50.98 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S495 (Residues: 1-443; Coverage: 100%)
Gene name: CTHT_0039380
Pfam: Nop53 (60S ribosomal biogenesis)

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Chain: Cf
Length: 350 amino acids
Theoretical weight: 39.93 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SF80 (Residues: 1-350; Coverage: 100%)
Gene name: CTHT_0061110
Pfam: Brix domain

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Chain: Cg
Length: 202 amino acids
Theoretical weight: 22.82 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SCH6 (Residues: 1-202; Coverage: 100%)
Gene name: CTHT_0057260
Pfam: Ribosome biogenesis regulatory protein (RRS1)

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Chain: Ch
Length: 517 amino acids
Theoretical weight: 57.62 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Chaetomium thermophilum var. thermophilum DSM 1495
UniProt:
  • Canonical: G0SC29 (Residues: 1-517; Coverage: 100%)
Gene name: CTHT_0055700
Pfam: WD domain, G-beta repeat

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Chain: Cz
Length: 123 amino acids
Theoretical weight: 14.71 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SCP3 (Residues: 1-123; Coverage: 100%)
Gene name: CTHT_0057940
Pfam: Cgr1 family

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Chain: LA
Length: 254 amino acids
Theoretical weight: 27.6 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S4P8 (Residues: 1-254; Coverage: 100%)
Gene name: CTHT_0023090
Pfam:

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Chain: LB
Length: 392 amino acids
Theoretical weight: 44.46 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0RXW1 (Residues: 1-392; Coverage: 100%)
Gene name: CTHT_0004490
Pfam: Ribosomal protein L3

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Chain: LC
Length: 365 amino acids
Theoretical weight: 39.44 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SFC3 (Residues: 1-365; Coverage: 100%)
Gene name: CTHT_0061540
Pfam:

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Chain: LD
Length: 304 amino acids
Theoretical weight: 35.03 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SEG2 (Residues: 1-304; Coverage: 100%)
Gene name: CTHT_0063640
Pfam:

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Chain: LE
Length: 200 amino acids
Theoretical weight: 22.13 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S0D6 (Residues: 1-200; Coverage: 100%)
Gene name: CTHT_0009640
Pfam: Ribosomal protein L6e

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Chain: LG
Length: 262 amino acids
Theoretical weight: 29.74 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SAJ9 (Residues: 1-262; Coverage: 100%)
Gene name: CTHT_0042550
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: LH
Length: 229 amino acids
Theoretical weight: 25.87 KDa
Source organism: Thermochaetoides thermophila DSM 1495

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Chain: LJ
Length: 173 amino acids
Theoretical weight: 20.12 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SHQ2 (Residues: 1-173; Coverage: 100%)
Gene name: CTHT_0072970
Pfam:

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Chain: LK
Length: 165 amino acids
Theoretical weight: 17.8 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SGH8 (Residues: 1-165; Coverage: 100%)
Gene name: CTHT_0066380
Pfam:

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Chain: LL
Length: 213 amino acids
Theoretical weight: 24.19 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S992 (Residues: 1-213; Coverage: 100%)
Gene name: CTHT_0045000
Pfam: Ribosomal protein L13e

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Chain: LM
Length: 142 amino acids
Theoretical weight: 16.11 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S1R0 (Residues: 1-142; Coverage: 100%)
Gene name: CTHT_0014490
Pfam: Ribosomal protein L14

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Chain: LN
Length: 203 amino acids
Theoretical weight: 24.31 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0RZ88 (Residues: 1-203; Coverage: 100%)
Gene name: CTHT_0002100
Pfam: Ribosomal L15

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Chain: LO
Length: 204 amino acids
Theoretical weight: 22.99 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SH61 (Residues: 1-204; Coverage: 100%)
Gene name: CTHT_0068840
Pfam: Ribosomal protein L13

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Chain: LP
Length: 187 amino acids
Theoretical weight: 21.07 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SGY1 (Residues: 1-187; Coverage: 100%)
Gene name: CTHT_0067970
Pfam: Ribosomal protein L22p/L17e

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Chain: LQ
Length: 213 amino acids
Theoretical weight: 24.2 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S9B5 (Residues: 1-213; Coverage: 100%)
Gene name: CTHT_0045230
Pfam: Ribosomal protein 60S L18 and 50S L18e

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Chain: LR
Length: 2898 amino acids
Theoretical weight: 317.09 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S9T3 (Residues: 1-2898; Coverage: 100%)
Gene name: CTHT_0047080
Pfam:

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Chain: LS
Length: 174 amino acids
Theoretical weight: 20.34 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SCE2 (Residues: 1-174; Coverage: 100%)
Gene name: CTHT_0056900
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

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Chain: LT
Length: 160 amino acids
Theoretical weight: 18.16 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S0Z3 (Residues: 1-160; Coverage: 100%)
Gene name: CTHT_0011760
Pfam: Ribosomal protein L21e

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Chain: LU
Length: 127 amino acids
Theoretical weight: 14.3 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SDS8 (Residues: 1-127; Coverage: 100%)
Gene name: CTHT_0052850
Pfam: Ribosomal L22e protein family

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Chain: LV
Length: 139 amino acids
Theoretical weight: 14.67 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SI44 (Residues: 1-139; Coverage: 100%)
Gene name: CTHT_0074440
Pfam: Ribosomal protein L14p/L23e

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Chain: LX
Length: 156 amino acids
Theoretical weight: 17.18 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S507 (Residues: 1-156; Coverage: 100%)
Gene name: CTHT_0023650
Pfam:

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Chain: LY
Length: 138 amino acids
Theoretical weight: 15.57 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0RYN9 (Residues: 1-138; Coverage: 100%)
Gene name: CTHT_0007360
Pfam:

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Chain: LZ
Length: 135 amino acids
Theoretical weight: 15.8 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0RZE9 (Residues: 1-135; Coverage: 100%)
Gene name: CTHT_0002720
Pfam: Ribosomal L27e protein family

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Chain: La
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Thermochaetoides thermophila DSM 1495

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Chain: Lc
Length: 108 amino acids
Theoretical weight: 11.6 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SEI0 (Residues: 1-108; Coverage: 100%)
Gene name: CTHT_0063820
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: Ld
Length: 120 amino acids
Theoretical weight: 13.87 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SD68 (Residues: 1-120; Coverage: 100%)
Gene name: CTHT_0059140
Pfam: Ribosomal protein L31e

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Chain: Le
Length: 131 amino acids
Theoretical weight: 15.1 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S6V4 (Residues: 1-131; Coverage: 100%)
Gene name: CTHT_0027000
Pfam: Ribosomal protein L32

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Chain: Lf
Length: 109 amino acids
Theoretical weight: 12.33 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SCL3 (Residues: 1-109; Coverage: 100%)
Gene name: CTHT_0057640
Pfam: Ribosomal protein L35Ae

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Chain: Lg
Length: 119 amino acids
Theoretical weight: 13.49 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SFN0 (Residues: 1-119; Coverage: 100%)
Gene name: CTHT_0071430
Pfam: Ribosomal protein L34e

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Chain: Lh
Length: 935 amino acids
Theoretical weight: 105.17 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S0D7 (Residues: 1-935; Coverage: 100%)
Gene name: CTHT_0009650
Pfam:

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Chain: Li
Length: 110 amino acids
Theoretical weight: 12.78 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0SBN1 (Residues: 1-110; Coverage: 100%)
Gene name: CTHT_0054170
Pfam: Ribosomal protein L36e

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Chain: Lj
Length: 95 amino acids
Theoretical weight: 10.66 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S101 (Residues: 1-95; Coverage: 100%)
Gene name: CTHT_0011850
Pfam: Ribosomal protein L37e

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Chain: Lk
Length: 94 amino acids
Theoretical weight: 10.9 KDa
Source organism: Thermochaetoides thermophila DSM 1495

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Chain: Ll
Length: 51 amino acids
Theoretical weight: 6.3 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: Q2H9R2 (Residues: 1-51; Coverage: 100%)
Gene name: CHGG_03042
Pfam: Ribosomal L39 protein

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Chain: Lp
Length: 92 amino acids
Theoretical weight: 10.16 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S6B0 (Residues: 1-92; Coverage: 100%)
Gene name: CTHT_0026110
Pfam: Ribosomal L37ae protein family

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Chain: Lr
Length: 217 amino acids
Theoretical weight: 24.06 KDa
Source organism: Thermochaetoides thermophila DSM 1495
UniProt:
  • Canonical: G0S4Z9 (Residues: 1-217; Coverage: 100%)
Gene name: CTHT_0023560
Pfam: Ribosomal protein L1p/L10e family

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Name: 26S rRNA
Representative chains: C1
Length: 3342 nucleotides
Theoretical weight: 1080.78 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: C2
Length: 156 nucleotides
Theoretical weight: 50.15 KDa
Rfam: 5.8S ribosomal RNA

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Name: ITS2
Representative chains: C3
Length: 162 nucleotides
Theoretical weight: 52.09 KDa

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Name: 5S ribosomal RNA
Representative chains: C4
Length: 119 nucleotides
Theoretical weight: 38.31 KDa
Rfam: 5S ribosomal RNA

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