› 8qel

X-ray diffraction
2.45Å resolution

PKR kinase domain- eIF2alpha in complex with compound

Released:
DOI: 10.2210/pdb8qel/pdb
PDB entry 8qel coloured by chain, front view.
PDB entry 8qel coloured by chain, side view.
PDB entry 8qel coloured by chain, top view.
Source organisms:
Entry authors: Nawrotek A, Vuillard L, Miallau L

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228501 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 2 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 20.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20459 (Residues: 4-176; Coverage: 57%)
Gene names: J1429, SUI2, TIF211, YJR007W
Sequence domains:
Interferon-induced, double-stranded RNA-activated protein kinase Chain: B
Molecule details ›
Chain: B
Length: 273 amino acids
Theoretical weight: 31.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19525 (Residues: 258-337, 344-541; Coverage: 48%)
Gene names: EIF2AK2, PKR, PRKR
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand UH3 1 x UH3
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3221
Unit cell:
a: 85.028Å b: 85.028Å c: 168.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.241 0.278
Expression system: Escherichia coli BL21(DE3)

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