Structure analysis

CrPhotLOV1 light state structure 7.5 ms (5-10 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature

X-ray diffraction
2.45Å resolution
Source organism: Chlamydomonas reinhardtii
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: monomeric
Accessible surface area: 6241.24 Å2
Buried surface area: 847.73 Å2
Dissociation area: 423.86 Å2
Dissociation energy (ΔGdiss): 4.63 kcal/mol
Dissociation entropy (TΔSdiss): 5.16 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-185298
Assembly 2
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Multimeric state: homo dimer
Accessible surface area: 10792.36 Å2
Buried surface area: 3387.16 Å2
Dissociation area: 846.57 Å2
Dissociation energy (ΔGdiss): 4.79 kcal/mol
Dissociation entropy (TΔSdiss): 11.29 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-185299

Macromolecules

Chain: A
Length: 138 amino acids
Theoretical weight: 15.34 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8LPD9 (Residues: 16-133; Coverage: 16%)
Gene names: CHLREDRAFT_183965, CHLRE_03g199000v5, PHOT
Pfam: PAS domain
PDBe-KB: UniProt Coverage View: Q8LPD9  
1138102030405060708090100110120130
 
50100HHHHHHHHHHSSGHIEGRHMAGLRHTFVVADATLPDCPLVYASEGFYAMTGYGPDEVLGHNCRFLQGEGTDPKEVQKIRDAIKKGEACSVRLLNYRKDGTPFWNLLTVTPIKTPDGRVSKFVGVQVDVTSKTEGKALA
UniProt
Q8LPD9
Chains
Secondary structure
Ligand binding sites
Sequence conservation

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