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CrPhotLOV1 light state structure 12.5 ms (10-15 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature

X-ray diffraction
2.45Å resolution
PDB entry 8qif coloured by chain, front view.
PDB entry 8qif coloured by chain, side view.
PDB entry 8qif coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Phototropin
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Multimeric state: monomeric
Accessible surface area: 6284.55 Å2
Buried surface area: 844.85 Å2
Dissociation area: 422.43 Å2
Dissociation energy (ΔGdiss): 4.45 kcal/mol
Dissociation entropy (TΔSdiss): 5.17 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-185298
Assembly 2
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Assembly Name: Phototropin
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Multimeric state: homo dimer
Accessible surface area: 10858.12 Å2
Buried surface area: 3404.14 Å2
Dissociation area: 857.98 Å2
Dissociation energy (ΔGdiss): 4.61 kcal/mol
Dissociation entropy (TΔSdiss): 11.29 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-185299

Macromolecules

Phototropin
Chain: A
Length: 138 amino acids
Theoretical weight: 15.34 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8LPD9 (Residues: 16-133; Coverage: 16%)
Gene names: CHLREDRAFT_183965, CHLRE_03g199000v5, PHOT
Pfam: PAS domain
Phototropin in PDB entry 8qif, assembly 1, front view.
Phototropin in PDB entry 8qif, assembly 1, side view.
Phototropin in PDB entry 8qif, assembly 1, top view.
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