› 8qqr

Electron Microscopy
2.73Å resolution

Mycobacterium smegmatis inosine monophosphate dehydrogenase (IMPDH) ATP+ppGpp-bound form, super-compressed

Released:
DOI: 10.2210/pdb8qqr/pdb
PDB entry 8qqr coloured by chain, front view.
PDB entry 8qqr coloured by chain, side view.
PDB entry 8qqr coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Deciphering the allosteric regulation of mycobacterial inosine-5'-monophosphate dehydrogenase.
Bulvas O, Knejzlík Z, Sýs J, Filimoněnko A, Čížková M, Clarová K, Rejman D, Kouba T, Pichová I
Nat Commun 15 6673 (2024)
PMID: 39107302
Related structures: EMD-18602

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 513 amino acids
Theoretical weight: 53.39 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0QSU3 (Residues: 1-513; Coverage: 100%)
Gene names: MSMEG_1602, guaB
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 8 x ATP
Ligand G4P 8 x G4P
Ligand MG 16 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.73Å
Relevant EMDB volumes: EMD-18602
Expression system: Escherichia coli BL21(DE3)

Quick links