› 8qx8

Electron Microscopy
4.6Å resolution

Endosomal membrane tethering complex CORVET

Released:
DOI: 10.2210/pdb8qx8/pdb
PDB entry 8qx8 coloured by chain, front view.
PDB entry 8qx8 coloured by chain, side view.
PDB entry 8qx8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the endosomal CORVET tethering complex.
Shvarev D, König C, Susan N, Langemeyer L, Walter S, Perz A, Fröhlich F, Ungermann C, Moeller A
OpenAccess logo Nat Commun 15 5227 (2024)
PMID: 38898033
Related structures: EMD-18701

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-273197 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Vacuolar protein sorting-associated protein 8 Chain: F
Molecule details ›
Chain: F
Length: 1298 amino acids
Theoretical weight: 148.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39702 (Residues: 1-1274; Coverage: 100%)
Gene names: FUN15, VPS8, VPT8, YAL002W
Sequence domains: Golgi CORVET complex core vacuolar protein 8
Vacuolar protein sorting-associated protein 33 Chain: D
Molecule details ›
Chain: D
Length: 691 amino acids
Theoretical weight: 79.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P20795 (Residues: 1-691; Coverage: 100%)
Gene names: L8084.15, SLP1, VAM5, VPS33, YLR396C
Sequence domains: Sec1 family
Vacuolar protein sorting-associated protein 16 Chain: B
E3 ubiquitin-protein ligase PEP5 Chain: A
Vacuolar protein sorting-associated protein 3 Chain: E
Vacuolar membrane protein PEP3 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-18701
Expression system: Saccharomyces cerevisiae

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