› 8qzz

X-ray diffraction
3.35Å resolution

Crystal structure of human eIF2 alpha-gamma complexed with PPP1R15A_420-452

Released:
DOI: 10.2210/pdb8qzz/pdb
PDB entry 8qzz coloured by chain, front view.
PDB entry 8qzz coloured by chain, side view.
PDB entry 8qzz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substrate recruitment via eIF2γ enhances catalytic efficiency of a holophosphatase that terminates the integrated stress response.
Yan Y, Shetty M, Harding HP, George G, Zyryanova A, Labbé K, Mafi A, Hao Q, Sidrauski C, Ron D
Proc Natl Acad Sci U S A 121 e2320013121 (2024)
PMID: 38547060

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-271865 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Eukaryotic translation initiation factor 2 subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 472 amino acids
Theoretical weight: 51.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41091 (Residues: 1-472; Coverage: 100%)
Gene names: EIF2G, EIF2S3
Sequence domains:
Eukaryotic translation initiation factor 2 subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 315 amino acids
Theoretical weight: 36.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05198 (Residues: 1-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:
Protein phosphatase 1 regulatory subunit 15A Chain: C
Molecule details ›
Chain: C
Length: 33 amino acids
Theoretical weight: 3.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75807 (Residues: 420-452; Coverage: 5%)
Gene names: GADD34, PPP1R15A

Ligands and Environments

1 bound ligand:
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P41212
Unit cell:
a: 120.594Å b: 120.594Å c: 158.259Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.241 0.299
Expression system: Escherichia coli

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