› 8r3g

Electron Microscopy
4.4Å resolution

Central glycolytic genes regulator (CggR) bound to DNA operator

Released:
DOI: 10.2210/pdb8r3g/pdb
PDB entry 8r3g coloured by chain, front view.
PDB entry 8r3g coloured by chain, side view.
PDB entry 8r3g coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function.
Šoltysová M, Škerlová J, Pachl P, Škubník K, Fábry M, Sieglová I, Farolfi M, Grishkovskaya I, Babiak M, Nováček J, Krásný L, Řezáčová P
OpenAccess logo Nucleic Acids Res (2024)
PMID: 38842936
Related structures: EMD-18864

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-513118 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Central glycolytic genes regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 346 amino acids
Theoretical weight: 38.62 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32253 (Residues: 1-340; Coverage: 100%)
Gene names: BSU33950, cggR, yvbQ
Sequence domains:
operator DNA Chain: F
Molecule details ›
Chain: F
Length: 45 nucleotides
Theoretical weight: 13.78 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
operator DNA Chain: E
Molecule details ›
Chain: E
Length: 45 nucleotides
Theoretical weight: 13.93 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-18864
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 mention without citation

Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function.
Šoltysová et al. (2024)