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Central glycolytic genes regulator (CggR) bound to DNA operator

Electron Microscopy
4.4Å resolution
PDB entry 8r3g coloured by chain, front view.
PDB entry 8r3g coloured by chain, side view.
PDB entry 8r3g coloured by chain, top view.
Source organism: Bacillus subtilis
Assembly composition:
hetero hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Central glycolytic genes regulator and DNA
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Multimeric state: hetero hexamer
Accessible surface area: 69747.21 Å2
Buried surface area: 17005.65 Å2
Dissociation area: 2,707.85 Å2
Dissociation energy (ΔGdiss): 26.78 kcal/mol
Dissociation entropy (TΔSdiss): 16.18 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-270694

Macromolecules

Central glycolytic genes regulator
Chains: A, B, C, D
Length: 346 amino acids
Theoretical weight: 38.62 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32253 (Residues: 1-340; Coverage: 100%)
Gene names: BSU33950, cggR, yvbQ
Pfam:
Central glycolytic genes regulator in PDB entry 8r3g, assembly 1, front view.
Central glycolytic genes regulator in PDB entry 8r3g, assembly 1, side view.
Central glycolytic genes regulator in PDB entry 8r3g, assembly 1, top view.
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DNA chain F
Name: operator DNA
Representative chains: F
Source organism: Bacillus subtilis [1423]
Expression system: Not provided
Length: 45 nucleotides
Theoretical weight: 13.78 KDa
operator DNA in PDB entry 8r3g, assembly 1, front view.
operator DNA in PDB entry 8r3g, assembly 1, side view.
operator DNA in PDB entry 8r3g, assembly 1, top view.
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DNA chain E
Name: operator DNA
Representative chains: E
Source organism: Bacillus subtilis [1423]
Expression system: Not provided
Length: 45 nucleotides
Theoretical weight: 13.93 KDa
operator DNA in PDB entry 8r3g, assembly 1, front view.
operator DNA in PDB entry 8r3g, assembly 1, side view.
operator DNA in PDB entry 8r3g, assembly 1, top view.
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