Structure analysis

Escherichia coli paused disome complex (non-rotated disome interface)

Electron Microscopy
3.28Å resolution
Source organism: Escherichia coli
Assembly composition:
hetero 65-mer (preferred)
Entry contents: 36 distinct polypeptide molecules
9 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 65-mer
PDBe Complex ID: PDB-CPX-240785

Macromolecules

Chain: 12
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M2 (Residues: 1-78; Coverage: 100%)
Gene names: JW3612, b3637, rpmB
Pfam: Ribosomal L28 family

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Chain: 32
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG51 (Residues: 1-59; Coverage: 100%)
Gene names: JW3264, b3302, rpmD
Pfam: Ribosomal protein L30p/L7e

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Chain: 4
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M9 (Residues: 1-70; Coverage: 100%)
Gene names: JW3907, b3936, rpmE
Pfam: Ribosomal protein L31

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Chain: 62
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q1 (Residues: 1-65; Coverage: 100%)
Gene names: JW1707, b1717, rpmI
Pfam: Ribosomal protein L35

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Chains: B, B2
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Pfam: Ribosomal protein S2

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Chains: C1, C2
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Pfam:

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Chains: D1, D2
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Pfam:

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Chains: E1, E2
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)
Gene names: JW3265, b3303, rpsE, spc
Pfam:

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Chains: F1, F2
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Pfam: Ribosomal protein S6

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Chains: G1, G2
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 1-179; Coverage: 100%)
Gene names: JW3303, b3341, rpsG
Pfam: Ribosomal protein S7p/S5e

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Chains: H1, H2
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Pfam: Ribosomal protein S8

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Chains: I1, I2
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 1-130; Coverage: 100%)
Gene names: JW3199, b3230, rpsI
Pfam: Ribosomal protein S9/S16

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Chains: J1, J2
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Pfam: Ribosomal protein S10p/S20e

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Chains: K1, K2
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 1-129; Coverage: 100%)
Gene names: JW3259, b3297, rpsK
Pfam: Ribosomal protein S11

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Chains: L1, L2
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Pfam: Ribosomal protein S12/S23

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Chains: M1, M2
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 1-118; Coverage: 100%)
Gene names: JW3260, b3298, rpsM
Pfam: Ribosomal protein S13/S18

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Chains: N1, N2
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 1-101; Coverage: 100%)
Gene names: JW3269, b3307, rpsN
Pfam: Ribosomal protein S14p/S29e

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Chains: O1, O2
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Pfam: Ribosomal protein S15

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Chain: P1
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Pfam: Ribosomal protein S16

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Chains: Q1, Q2
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Pfam: Ribosomal protein S17

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Chains: R1, R2
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 1-75; Coverage: 100%)
Gene names: JW4160, b4202, rpsR
Pfam: Ribosomal protein S18

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Chains: S1, S2
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 1-92; Coverage: 100%)
Gene names: JW3278, b3316, rpsS
Pfam: Ribosomal protein S19

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Chain: T1
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Pfam: Ribosomal protein S20

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Chains: U1, U2
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Pfam: Ribosomal protein S21

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Chain: Z1
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG67 (Residues: 1-557; Coverage: 100%)
Gene names: JW0894, b0911, rpsA, ssyF
Pfam: S1 RNA binding domain

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Chain: a2
Length: 234 amino acids
Theoretical weight: 24.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L0 (Residues: 1-234; Coverage: 100%)
Gene names: JW3947, b3984, rplA
Pfam: Ribosomal protein L1p/L10e family

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Chain: b2
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Pfam:

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Chain: e2
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P62399 (Residues: 1-179; Coverage: 100%)
Gene names: JW3270, b3308, rplE
Pfam:

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Chain: g2
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N9 (Residues: 1-55; Coverage: 100%)
Gene names: JW3611, b3636, rpmG
Pfam: Ribosomal protein L33

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Chain: h2
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Pfam: Ribosomal protein L16p/L10e

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Chain: i2
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R1 (Residues: 1-149; Coverage: 100%)
Gene names: JW4161, b4203, rplI
Pfam:

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Chain: l2
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Pfam: Ribosomal protein L34

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Chain: o2
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: r2
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Gene names: JW3266, b3304, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

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Chain: z2
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Gene names: JW3152, b3185, rpmA
Pfam: Ribosomal L27 protein

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Name: 23S ribosomal RNA
Representative chains: 71, 72
Length: 2904 nucleotides
Theoretical weight: 941.81 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: 82
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Rfam: 5S ribosomal RNA

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Name: 16S ribosomal RNA
Representative chains: A1, A2
Length: 1542 nucleotides
Theoretical weight: 499.87 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: Messenger RNA
Representative chains: V2
Length: 64 nucleotides
Theoretical weight: 20.79 KDa

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Name: tRNA-Trp (P-site)
Representative chains: W
Length: 76 nucleotides
Theoretical weight: 24.63 KDa
Rfam: tRNA

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Name: tRNA-Phe (P-site)
Representative chains: W1
Length: 76 nucleotides
Theoretical weight: 24.65 KDa
Rfam: tRNA

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Name: tRNA-Arg (E-site)
Representative chains: X2
Length: 77 nucleotides
Theoretical weight: 24.98 KDa
Rfam: tRNA

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Name: tRNA-Val (A-site)
Representative chains: Y1
Length: 76 nucleotides
Theoretical weight: 24.62 KDa
Rfam: tRNA

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Name: tRNA-Ala (A-site)
Representative chains: Y2
Length: 76 nucleotides
Theoretical weight: 24.56 KDa
Rfam: tRNA

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