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Plastid-encoded RNA polymerase

Electron Microscopy
2.49Å resolution
PDB entry 8r5o coloured by chain, front view.
PDB entry 8r5o coloured by chain, side view.
PDB entry 8r5o coloured by chain, top view.
Source organism: Sinapis alba
Assembly composition:
hetero icosamer (preferred)
Entry contents: 18 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero icosamer
Accessible surface area: 260511.99 Å2
Buried surface area: 136057.85 Å2
Dissociation area: 1,392.42 Å2
Dissociation energy (ΔGdiss): 6.44 kcal/mol
Dissociation entropy (TΔSdiss): 15.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-241099

Macromolecules

DNA-directed RNA polymerase subunit alpha
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.95 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: A0A6C0M610 (Residues: 1-327; Coverage: 100%)
Gene name: rpoA
Pfam:
DNA-directed RNA polymerase subunit alpha in PDB entry 8r5o, assembly 1, front view.
DNA-directed RNA polymerase subunit alpha in PDB entry 8r5o, assembly 1, side view.
DNA-directed RNA polymerase subunit alpha in PDB entry 8r5o, assembly 1, top view.
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DNA-directed RNA polymerase subunit beta
Chain: C
Length: 1072 amino acids
Theoretical weight: 121.21 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: A0A6C0M5W1 (Residues: 1-1072; Coverage: 100%)
Gene name: rpoB
Pfam:
DNA-directed RNA polymerase subunit beta in PDB entry 8r5o, assembly 1, front view.
DNA-directed RNA polymerase subunit beta in PDB entry 8r5o, assembly 1, side view.
DNA-directed RNA polymerase subunit beta in PDB entry 8r5o, assembly 1, top view.
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DNA-directed RNA polymerase subunit beta'
Chain: D
Length: 680 amino acids
Theoretical weight: 78.76 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: A0A6C0M5W0 (Residues: 1-680; Coverage: 100%)
Gene name: rpoC1
Pfam:
DNA-directed RNA polymerase subunit beta' in PDB entry 8r5o, assembly 1, front view.
DNA-directed RNA polymerase subunit beta' in PDB entry 8r5o, assembly 1, side view.
DNA-directed RNA polymerase subunit beta' in PDB entry 8r5o, assembly 1, top view.
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DNA-directed RNA polymerase subunit beta''
Chain: E
Length: 1373 amino acids
Theoretical weight: 156.39 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: A0A6C0M829 (Residues: 1-1373; Coverage: 100%)
Gene name: rpoC2
Pfam:
DNA-directed RNA polymerase subunit beta'' in PDB entry 8r5o, assembly 1, front view.
DNA-directed RNA polymerase subunit beta'' in PDB entry 8r5o, assembly 1, side view.
DNA-directed RNA polymerase subunit beta'' in PDB entry 8r5o, assembly 1, top view.
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PAP1
Chain: F
Length: 911 amino acids
Theoretical weight: 103.47 KDa
Source organism: Sinapis alba
PAP1 in PDB entry 8r5o, assembly 1, front view.
PAP1 in PDB entry 8r5o, assembly 1, side view.
PAP1 in PDB entry 8r5o, assembly 1, top view.
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PAP2
Chain: G
Length: 862 amino acids
Theoretical weight: 96.11 KDa
Source organism: Sinapis alba
PAP2 in PDB entry 8r5o, assembly 1, front view.
PAP2 in PDB entry 8r5o, assembly 1, side view.
PAP2 in PDB entry 8r5o, assembly 1, top view.
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PAP3
Chain: H
Length: 675 amino acids
Theoretical weight: 79.82 KDa
Source organism: Sinapis alba
PAP3 in PDB entry 8r5o, assembly 1, front view.
PAP3 in PDB entry 8r5o, assembly 1, side view.
PAP3 in PDB entry 8r5o, assembly 1, top view.
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PAP4
Chain: I
Length: 263 amino acids
Theoretical weight: 30.27 KDa
Source organism: Sinapis alba
PAP4 in PDB entry 8r5o, assembly 1, front view.
PAP4 in PDB entry 8r5o, assembly 1, side view.
PAP4 in PDB entry 8r5o, assembly 1, top view.
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PAP5
Chain: J
Length: 529 amino acids
Theoretical weight: 60.88 KDa
Source organism: Sinapis alba
PAP5 in PDB entry 8r5o, assembly 1, front view.
PAP5 in PDB entry 8r5o, assembly 1, side view.
PAP5 in PDB entry 8r5o, assembly 1, top view.
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PAP6
Chain: K
Length: 460 amino acids
Theoretical weight: 52.44 KDa
Source organism: Sinapis alba
PAP6 in PDB entry 8r5o, assembly 1, front view.
PAP6 in PDB entry 8r5o, assembly 1, side view.
PAP6 in PDB entry 8r5o, assembly 1, top view.
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PAP7
Chain: L
Length: 483 amino acids
Theoretical weight: 55.68 KDa
Source organism: Sinapis alba
PAP7 in PDB entry 8r5o, assembly 1, front view.
PAP7 in PDB entry 8r5o, assembly 1, side view.
PAP7 in PDB entry 8r5o, assembly 1, top view.
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PAP8
Chain: M
Length: 334 amino acids
Theoretical weight: 38.04 KDa
Source organism: Sinapis alba
PAP8 in PDB entry 8r5o, assembly 1, front view.
PAP8 in PDB entry 8r5o, assembly 1, side view.
PAP8 in PDB entry 8r5o, assembly 1, top view.
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PAP9
Chain: N
Length: 297 amino acids
Theoretical weight: 34.01 KDa
Source organism: Sinapis alba
PAP9 in PDB entry 8r5o, assembly 1, front view.
PAP9 in PDB entry 8r5o, assembly 1, side view.
PAP9 in PDB entry 8r5o, assembly 1, top view.
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PAP10
Chains: O, P
Length: 185 amino acids
Theoretical weight: 20.95 KDa
Source organism: Sinapis alba
PAP10 in PDB entry 8r5o, assembly 1, front view.
PAP10 in PDB entry 8r5o, assembly 1, side view.
PAP10 in PDB entry 8r5o, assembly 1, top view.
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PAP11
Chain: Q
Length: 768 amino acids
Theoretical weight: 85.12 KDa
Source organism: Sinapis alba
PAP11 in PDB entry 8r5o, assembly 1, front view.
PAP11 in PDB entry 8r5o, assembly 1, side view.
PAP11 in PDB entry 8r5o, assembly 1, top view.
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PAP12
Chain: R
Length: 162 amino acids
Theoretical weight: 18.84 KDa
Source organism: Sinapis alba
PAP12 in PDB entry 8r5o, assembly 1, front view.
PAP12 in PDB entry 8r5o, assembly 1, side view.
PAP12 in PDB entry 8r5o, assembly 1, top view.
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FLN2
Chain: S
Length: 611 amino acids
Theoretical weight: 68.53 KDa
Source organism: Sinapis alba
FLN2 in PDB entry 8r5o, assembly 1, front view.
FLN2 in PDB entry 8r5o, assembly 1, side view.
FLN2 in PDB entry 8r5o, assembly 1, top view.
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PTAC18
Chain: T
Length: 140 amino acids
Theoretical weight: 16.43 KDa
Source organism: Sinapis alba
PTAC18 in PDB entry 8r5o, assembly 1, front view.
PTAC18 in PDB entry 8r5o, assembly 1, side view.
PTAC18 in PDB entry 8r5o, assembly 1, top view.
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