› 8r60

Electron Microscopy
3.23Å resolution

1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide

Released:
DOI: 10.2210/pdb8r60/pdb
PDB entry 8r60 coloured by chain, front view.
PDB entry 8r60 coloured by chain, side view.
PDB entry 8r60 coloured by chain, top view.
Source organisms:
Entry authors: Keown JR, Carrique L, Fodor E, Grimes JM
Related structures: EMD-18945

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-245775 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 82.66 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3HM39 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 905 amino acids
Theoretical weight: 102.38 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3HM41 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Sequence domains:
DNA-directed RNA polymerase II subunit RPB1 Chain: X
Molecule details ›
Chain: X
Length: 28 amino acids
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24928 (Residues: 1713-1740; Coverage: 1%)
Gene names: POLR2, POLR2A
Sequence domains: RNA polymerase Rpb1 C-terminal repeat
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 757 amino acids
Theoretical weight: 86.63 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3HM40 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.86 KDa
RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.34 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.23Å
Relevant EMDB volumes: EMD-18945
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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