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1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide

Electron Microscopy
3.23Å resolution
PDB entry 8r60 coloured by chain, front view.
PDB entry 8r60 coloured by chain, side view.
PDB entry 8r60 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 4 distinct polypeptide molecules
2 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 67162.72 Å2
Buried surface area: 39477.22 Å2
Dissociation area: 1,095.02 Å2
Dissociation energy (ΔGdiss): 10.85 kcal/mol
Dissociation entropy (TΔSdiss): 9.51 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-279872

Macromolecules

Polymerase acidic protein
Chain: A
Length: 716 amino acids
Theoretical weight: 82.66 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3HM39 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Pfam: Influenza RNA-dependent RNA polymerase subunit PA
Polymerase acidic protein in PDB entry 8r60, assembly 1, front view.
Polymerase acidic protein in PDB entry 8r60, assembly 1, side view.
Polymerase acidic protein in PDB entry 8r60, assembly 1, top view.
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Polymerase basic protein 2
Chain: C
Length: 905 amino acids
Theoretical weight: 102.38 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3HM41 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Pfam:
Polymerase basic protein 2 in PDB entry 8r60, assembly 1, front view.
Polymerase basic protein 2 in PDB entry 8r60, assembly 1, side view.
Polymerase basic protein 2 in PDB entry 8r60, assembly 1, top view.
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DNA-directed RNA polymerase II subunit RPB1
Chain: X
Length: 28 amino acids
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24928 (Residues: 1713-1740; Coverage: 1%)
Gene names: POLR2, POLR2A
Pfam: RNA polymerase Rpb1 C-terminal repeat
RNA polymerase II 4 repeat peptide with serine5 phosphorylation in PDB entry 8r60, assembly 1, front view.
RNA polymerase II 4 repeat peptide with serine5 phosphorylation in PDB entry 8r60, assembly 1, side view.
RNA polymerase II 4 repeat peptide with serine5 phosphorylation in PDB entry 8r60, assembly 1, top view.
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RNA-directed RNA polymerase catalytic subunit
Chain: B
Length: 757 amino acids
Theoretical weight: 86.63 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q3HM40 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Pfam: Influenza RNA-dependent RNA polymerase subunit PB1
RNA-directed RNA polymerase catalytic subunit in PDB entry 8r60, assembly 1, front view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 8r60, assembly 1, side view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 8r60, assembly 1, top view.
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RNA chain D
Name: RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3')
Representative chains: D
Length: 15 nucleotides
Theoretical weight: 4.86 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') in PDB entry 8r60, assembly 1, front view.
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') in PDB entry 8r60, assembly 1, side view.
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') in PDB entry 8r60, assembly 1, top view.
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RNA chain E
Name: RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3')
Representative chains: E
Length: 17 nucleotides
Theoretical weight: 5.34 KDa
RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') in PDB entry 8r60, assembly 1, front view.
RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') in PDB entry 8r60, assembly 1, side view.
RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') in PDB entry 8r60, assembly 1, top view.
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