› 8rbz

Electron Microscopy
3.7Å resolution

Structure of Integrator-PP2A-SOSS-CTD post-termination complex

Released:
DOI: 10.2210/pdb8rbz/pdb
PDB entry 8rbz coloured by chain, front view.
PDB entry 8rbz coloured by chain, side view.
PDB entry 8rbz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Integrator-dependent RNA polymerase II termination.
Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P
Nature (2024)
PMID: 38570683
Related structures: EMD-19040

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239616 (preferred)
Entry contents:
21 distinct polypeptide molecules
Macromolecules (21 distinct):
UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK Chain: 1
Molecule details ›
Chain: 1
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Sus scrofa
SOSS complex subunit B1 Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 22.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BQ15 (Residues: 1-211; Coverage: 100%)
Gene names: LP3587, NABP2, OBFC2B, SSB1
Sequence domains: Single-stranded DNA binding protein Ssb-like, OB fold
SOSS complex subunit C Chain: C
Molecule details ›
Chain: C
Length: 106 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NRY2 (Residues: 1-104; Coverage: 100%)
Gene names: C9orf80, HSPC043, HSPC291, INIP, SSBIP1
Sequence domains: SOSS complex subunit C
DNA-directed RNA polymerase subunit Chain: Y
Molecule details ›
Chain: Y
Length: 13 amino acids
Theoretical weight: 1.36 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LJR4 (Residues: 1792-1804; Coverage: 1%)
Gene name: POLR2A
Sequence domains: RNA polymerase Rpb1 C-terminal repeat
Integrator complex subunit 1 Chain: a
Molecule details ›
Chain: a
Length: 2192 amino acids
Theoretical weight: 244.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8N201 (Residues: 1-2190; Coverage: 100%)
Gene names: INTS1, KIAA1440, UNQ1821/PRO3434
Sequence domains:
Integrator complex subunit 2 Chain: b
Molecule details ›
Chain: b
Length: 1204 amino acids
Theoretical weight: 134.45 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H0H0 (Residues: 1-1204; Coverage: 100%)
Gene names: INTS2, KIAA1287
Sequence domains: Integrator complex subunit 2
Integrator complex subunit 3 Chain: c
Molecule details ›
Chain: c
Length: 1042 amino acids
Theoretical weight: 118.16 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q68E01 (Residues: 1-1043; Coverage: 100%)
Gene names: C1orf193, C1orf60, INTS3
Sequence domains: Integrator complex subunit 3 N-terminal
Integrator complex subunit 4 Chain: d
Molecule details ›
Chain: d
Length: 963 amino acids
Theoretical weight: 108.31 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96HW7 (Residues: 1-963; Coverage: 100%)
Gene names: INTS4, MSTP093
Sequence domains:
Integrator complex subunit 5 Chain: e
Molecule details ›
Chain: e
Length: 1021 amino acids
Theoretical weight: 108.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia
UniProt:
  • Canonical: Q6P9B9 (Residues: 1-1019; Coverage: 100%)
Gene names: INTS5, KIAA1698
Sequence domains:
Integrator complex subunit 6 Chain: f
Molecule details ›
Chain: f
Length: 889 amino acids
Theoretical weight: 100.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UL03 (Residues: 1-887; Coverage: 100%)
Gene names: DBI1, DDX26, DDX26A, INTS6
Sequence domains:
Integrator complex subunit 7 Chain: g
Molecule details ›
Chain: g
Length: 964 amino acids
Theoretical weight: 107.15 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NVH2 (Residues: 1-962; Coverage: 100%)
Gene names: C1orf73, INTS7
Sequence domains: Integrator complex subunit 7, C-terminal domain
Integrator complex subunit 8 Chain: h
Molecule details ›
Chain: h
Length: 995 amino acids
Theoretical weight: 113.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q75QN2 (Residues: 1-995; Coverage: 100%)
Gene names: C8orf52, INTS8
Integrator complex subunit 9 Chain: i
Molecule details ›
Chain: i
Length: 658 amino acids
Theoretical weight: 73.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NV88 (Residues: 1-658; Coverage: 100%)
Gene names: INTS9, RC74
Sequence domains:
Integrator complex subunit 10 Chain: j
Molecule details ›
Chain: j
Length: 710 amino acids
Theoretical weight: 82.34 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NVR2 (Residues: 1-710; Coverage: 100%)
Gene names: C8orf35, INTS10
Sequence domains: Integrator subunit 10
Integrator complex subunit 11 Chain: k
Molecule details ›
Chain: k
Length: 602 amino acids
Theoretical weight: 67.97 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5TA45 (Residues: 1-600; Coverage: 100%)
Gene names: CPSF3L, INTS11, RC68
Sequence domains:
Integrator complex subunit 14 Chain: n
Molecule details ›
Chain: n
Length: 518 amino acids
Theoretical weight: 57.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96SY0 (Residues: 1-518; Coverage: 100%)
Gene names: C15orf44, INTS14, VWA9
Sequence domains:
Integrator complex subunit 15 Chain: o
Molecule details ›
Chain: o
Length: 451 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96N11 (Residues: 1-449; Coverage: 100%)
Gene names: C7orf26, INTS15
Sequence domains: Integrator complex subunit 15
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: p
Molecule details ›
Chain: p
Length: 591 amino acids
Theoretical weight: 65.58 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30153 (Residues: 1-589; Coverage: 100%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: q
Molecule details ›
Chain: q
Length: 311 amino acids
Theoretical weight: 35.84 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
DSS1 Chain: r
Molecule details ›
Chain: r
Length: 27 amino acids
Theoretical weight: 3.38 KDa
Source organism: Trichoplusia ni
Integrator complex subunit 13 Chain: m
Molecule details ›
Chain: m
Length: 706 amino acids
Theoretical weight: 80.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NVM9 (Residues: 1-706; Coverage: 100%)
Gene names: ASUN, C12orf11, GCT1, INTS13
Sequence domains: Cell cycle and development regulator Mat89Bb

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-19040
Expression systems:
  • Trichoplusia ni
  • Trichoplusia

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