› 8rpb

X-ray diffraction
2.79Å resolution

Structure of S79 Fab in complex with IgV domain of human PD-L1

Released:
DOI: 10.2210/pdb8rpb/pdb
PDB entry 8rpb coloured by chain, front view.
PDB entry 8rpb coloured by chain, side view.
PDB entry 8rpb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of light chain-driven bispecific antibodies targeting CD47 and PD-L1.
Malinge P, Chauchet X, Bourguignon J, Bosson N, Calloud S, Bautzova T, Borlet M, Laursen M, Kelpsas V, Rose N, Gueneau F, Ravn U, Magistrelli G, Fischer N
MAbs 16 2362432 (2024)
PMID: 38849989

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246103 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
heavy chain of S79 fab Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
light chain of S79 fab Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Programmed cell death 1 ligand 1 Chain: P
Molecule details ›
Chain: P
Length: 125 amino acids
Theoretical weight: 14.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZQ7 (Residues: 18-133; Coverage: 42%)
Gene names: B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Sequence domains: Immunoglobulin V-set domain

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand GOL 3 x GOL
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P6222
Unit cell:
a: 185.852Å b: 185.852Å c: 110.237Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.229 0.251
Expression systems:
  • Homo sapiens
  • Escherichia coli

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