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Rap from bacteriophage Phi3T in presence of pheromone RGHTS

X-ray diffraction
2.83Å resolution
PDB entry 8rtc coloured by chain, front view.
PDB entry 8rtc coloured by chain, side view.
PDB entry 8rtc coloured by chain, top view.
Source organism: Bacillus phage phi3T
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Rap3T and peptide
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Multimeric state: hetero dimer
Accessible surface area: 16498.48 Å2
Buried surface area: 1163.39 Å2
Dissociation area: 581.7 Å2
Dissociation energy (ΔGdiss): 1.18 kcal/mol
Dissociation entropy (TΔSdiss): 5.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-247239
Assembly 2 (preferred)
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Assembly Name: Rap3T and peptide
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Multimeric state: hetero dimer
Accessible surface area: 16661.18 Å2
Buried surface area: 1134.5 Å2
Dissociation area: 567.25 Å2
Dissociation energy (ΔGdiss): 3.02 kcal/mol
Dissociation entropy (TΔSdiss): 5.77 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-247239

Macromolecules

Rap3T
Chains: A, B
Length: 379 amino acids
Theoretical weight: 45.03 KDa
Source organism: Bacillus phage phi3T
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1P8CWN8 (Residues: 1-379; Coverage: 100%)
Gene name: phi3T_14
Pfam:
Rap3T in PDB entry 8rtc, assembly 2, front view.
Rap3T in PDB entry 8rtc, assembly 2, side view.
Rap3T in PDB entry 8rtc, assembly 2, top view.
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Pheromone RGHTS
Chains: C, D
Length: 5 amino acids
Theoretical weight: 559 Da
Source organism: Bacillus phage phi3T
Expression system: Not provided
Pheromone RGHTS in PDB entry 8rtc, assembly 2, front view.
Pheromone RGHTS in PDB entry 8rtc, assembly 2, side view.
Pheromone RGHTS in PDB entry 8rtc, assembly 2, top view.
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