› 8shu

X-ray diffraction
1.71Å resolution

Structure of mouse cGAS

Released:
DOI: 10.2210/pdb8shu/pdb
PDB entry 8shu coloured by chain, front view.
PDB entry 8shu coloured by chain, side view.
PDB entry 8shu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structural basis for 2'-5'/3'-5'-cGAMP synthesis by cGAS.
Wu S, Gabelli SB, Sohn J
OpenAccess logo Nat Commun 15 4012 (2024)
PMID: 38740774

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: C
Molecule details ›
Chain: C
Length: 364 amino acids
Theoretical weight: 42.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8C6L5 (Residues: 147-507; Coverage: 71%)
Gene names: Cgas, Mb21d1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Unit cell:
a: 87.892Å b: 61.054Å c: 105.704Å
α: 90° β: 94.57° γ: 90°
R-values:
R R work R free
0.179 0.177 0.211
Expression system: Escherichia coli BL21(DE3)

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