8sno

Electron Microscopy
2.78Å resolution

Structure of mature human ADAM17/iRhom2 sheddase complex, conformation 2

Released:

Function and Biology Details

Reaction catalysed:
Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-282875 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Disintegrin and metalloproteinase domain-containing protein 17 Chain: A
Molecule details ›
Chain: A
Length: 610 amino acids
Theoretical weight: 68.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P78536 (Residues: 215-824; Coverage: 76%)
Gene names: ADAM17, CSVP, TACE
Sequence domains:
Inactive rhomboid protein 2 Chain: B
Molecule details ›
Chain: B
Length: 827 amino acids
Theoretical weight: 93.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6PJF5 (Residues: 1-856; Coverage: 97%)
  • Best match: Q6PJF5-2 (Residues: 1-827)
Gene names: IRHOM2, RHBDF2, RHBDL5, RHBDL6
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.78Å
Relevant EMDB volumes: EMD-40631
Expression system: Homo sapiens