› 8sok

Electron Microscopy
4.1Å resolution

Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the presence of telomeric ssDNA

Released:
DOI: 10.2210/pdb8sok/pdb
PDB entry 8sok coloured by chain, front view.
PDB entry 8sok coloured by chain, side view.
PDB entry 8sok coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of CST-Polα/Primase recruitment and regulation by POT1 at telomeres.
Cai SW, Takai H, Walz T, de Lange T
bioRxiv (2023)
PMID: 37215005
Related structures: EMD-40660

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-231598 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (6 distinct):
CST complex subunit CTC1; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 1613 amino acids
Theoretical weight: 178.3 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q2NKJ3 (Residues: 1-1217; Coverage: 100%)
Gene names: C17orf68, CTC1, JW3994, b4034, malE
Sequence domains:
CST complex subunit STN1 Chain: B
Molecule details ›
Chain: B
Length: 368 amino acids
Theoretical weight: 42.17 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H668 (Residues: 1-368; Coverage: 100%)
Gene names: OBFC1, STN1
Sequence domains:
CST complex subunit TEN1 Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q86WV5 (Residues: 1-123; Coverage: 100%)
Gene names: C17orf106, TEN1
Sequence domains: Telomere-capping, CST complex subunit
Protection of telomeres protein 1 Chain: D
Molecule details ›
Chain: D
Length: 646 amino acids
Theoretical weight: 72.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NUX5 (Residues: 2-320, 321-634; Coverage: 100%)
Gene name: POT1
Sequence domains:
Adrenocortical dysplasia protein homolog Chain: E
Molecule details ›
Chain: E
Length: 529 amino acids
Theoretical weight: 58.06 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: B6F2F5 (Residues: 2-241; Coverage: 10%)
  • Canonical: Q96AP0 (Residues: 1-251; Coverage: 55%)
Gene names: ACD, PIP1, PTOP, TINT1, TPP1
Sequence domains:
DNA (5'-D(*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 18 nucleotides
Theoretical weight: 5.68 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-40660
Expression system: Trichoplusia ni

Quick links

Citations

1 mention without citation

Structural basis of CST-Polα/Primase recruitment and regulation by POT1 at telomeres.
Cai et al. (2023)