› 8szf

Electron Microscopy
2.8Å resolution

Cryo-EM structure of cinacalcet-bound active-state human calcium-sensing receptor CaSR in lipid nanodiscs

Released:
DOI: 10.2210/pdb8szf/pdb
PDB entry 8szf coloured by chain, front view.
PDB entry 8szf coloured by chain, side view.
PDB entry 8szf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric modulation and G-protein selectivity of the Ca2+-sensing receptor.
He F, Wu CG, Gao Y, Rahman SN, Zaoralová M, Papasergi-Scott MM, Gu TJ, Robertson MJ, Seven AB, Li L, Mathiesen JM, Skiniotis G
Nature (2024)
PMID: 38326620
Related structures: EMD-40914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239680 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Extracellular calcium-sensing receptor Chain: A
Molecule details ›
Chain: A
Length: 1101 amino acids
Theoretical weight: 123.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41180 (Residues: 19-894; Coverage: 83%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:
Extracellular calcium-sensing receptor Chain: B
Molecule details ›
Chain: B
Length: 959 amino acids
Theoretical weight: 108.02 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41180 (Residues: 19-894; Coverage: 83%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
6 bound ligands:
Ligand NAG 5 x NAG
Ligand TRP 2 x TRP
Ligand CA 4 x CA
Ligand PO4 2 x PO4
Ligand YP4 2 x YP4
Ligand SPM 1 x SPM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-40914
Expression system: Spodoptera frugiperda

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