› 8t20

Electron Microscopy
3.36Å resolution

Cryo-EM structure of mink variant Y453F trimeric spike protein bound to two mink ACE2 receptors

Released:
DOI: 10.2210/pdb8t20/pdb
PDB entry 8t20 coloured by chain, front view.
PDB entry 8t20 coloured by chain, side view.
PDB entry 8t20 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the American mink ACE2 binding by Y453F trimeric spike glycoproteins of SARS-CoV-2.
Ahn H, Calderon BM, Fan X, Gao Y, Horgan NL, Jiang N, Blohm DS, Hossain J, Rayyan NWK, Osman SH, Lin X, Currier M, Steel J, Wentworth DE, Zhou B, Liang B
J Med Virol 95 e29163 (2023)
PMID: 37842796
Related structures: EMD-40976

Function and Biology Details

Reaction catalysed: 
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232597 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1269 amino acids
Theoretical weight: 140.24 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
Angiotensin-converting enzyme Chains: D, E
Molecule details ›
Chains: D, E
Length: 771 amino acids
Theoretical weight: 89.38 KDa
Source organism: Neogale vison
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A7T0Q2W2 (Residues: 1-739; Coverage: 92%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.36Å
Relevant EMDB volumes: EMD-40976
Expression system: Homo sapiens

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