› 8tko

Electron Microscopy
3.05Å resolution

KS-AT core of 6-deoxyerythronolide B synthase (DEBS) Module 3 crosslinked with its translocation ACP partner of Module 2

Released:
DOI: 10.2210/pdb8tko/pdb
PDB entry 8tko coloured by chain, front view.
PDB entry 8tko coloured by chain, side view.
PDB entry 8tko coloured by chain, top view.
Source organism: Saccharopolyspora erythraea
Primary publication:
Structural basis for intermodular communication in assembly-line polyketide biosynthesis.
Cogan DP, Soohoo AM, Chen M, Liu Y, Brodsky KL, Khosla C
Nat Chem Biol (2024)
PMID: 39179672
Related structures: EMD-41355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269825 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
EryAII Erythromycin polyketide synthase modules 3 and 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 941 amino acids
Theoretical weight: 99.77 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4F7P0 (Residues: 2-922; Coverage: 26%)
Gene names: SACE_0723, eryAII
Sequence domains:
EryAI Erythromycin polyketide synthase modules 1 and 2 Chain: C
Molecule details ›
Chain: C
Length: 195 amino acids
Theoretical weight: 20.86 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4F7N8 (Residues: 3372-3545; Coverage: 5%)
Gene names: SACE_0721, eryAI
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 4HH 1 x 4HH

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-41355
Expression system: Escherichia coli BL21(DE3)

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