› 8ttb

Electron Microscopy
2.77Å resolution

Cryo-EM structure of the PP2A:B55-ARPP19 complex

Released:
DOI: 10.2210/pdb8ttb/pdb
PDB entry 8ttb coloured by chain, front view.
PDB entry 8ttb coloured by chain, side view.
PDB entry 8ttb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
Padi SKR, Vos MR, Godek RJ, Fuller JR, Kruse T, Hein JB, Nilsson J, Kelker MS, Page R, Peti W
OpenAccess logo Nature (2023)
PMID: 38123684
Related structures: EMD-41604

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-232849 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 584 amino acids
Theoretical weight: 64.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30153 (Residues: 9-589; Coverage: 99%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform Chain: B
Molecule details ›
Chain: B
Length: 450 amino acids
Theoretical weight: 52.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63151 (Residues: 2-447; Coverage: 100%)
Gene name: PPP2R2A
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 311 amino acids
Theoretical weight: 35.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
cAMP-regulated phosphoprotein 19 Chain: D
Molecule details ›
Chain: D
Length: 114 amino acids
Theoretical weight: 12.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P56211 (Residues: 1-112; Coverage: 100%)
Gene name: ARPP19
Sequence domains: cAMP-regulated phosphoprotein/endosulfine conserved region

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand ZN 1 x ZN
2 modified residues:
Ligand MLL 1 x MLL
Ligand 2RX 1 x 2RX

Experiments and Validation Details

Entry percentile scores
Resolution: 2.77Å
Relevant EMDB volumes: EMD-41604
Expression systems:
  • Escherichia coli
  • Homo sapiens

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