› 8ttf

Electron Microscopy
3.61Å resolution

NorA double mutant - E222QD307N at pH 7.5

Released:
DOI: 10.2210/pdb8ttf/pdb
PDB entry 8ttf coloured by chain, front view.
PDB entry 8ttf coloured by chain, side view.
PDB entry 8ttf coloured by chain, top view.
Source organisms:
Primary publication:
Proton-coupled transport mechanism of the efflux pump NorA.
Li J, Li Y, Koide A, Kuang H, Torres VJ, Koide S, Wang DN, Traaseth NJ
OpenAccess logo Nat Commun 15 4494 (2024)
PMID: 38802368
Related structures: EMD-41606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244869 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Quinolone resistance protein NorA Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 46.65 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A0J7 (Residues: 1-388; Coverage: 100%)
Gene name: norA
Sequence domains: Major Facilitator Superfamily
Heavy Chain of FabDA1 Variable Domain Chain: C
Molecule details ›
Chain: C
Length: 128 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Light Chain of FabDA1 Variable Domain Chain: D
Molecule details ›
Chain: D
Length: 105 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.61Å
Relevant EMDB volumes: EMD-41606
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Dynamics underlie the drug recognition mechanism by the efflux transporter EmrE.
Li et al. (2024)