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NorA double mutant - E222QD307N at pH 7.5

Electron Microscopy
3.61Å resolution
PDB entry 8ttf coloured by chain, front view.
PDB entry 8ttf coloured by chain, side view.
PDB entry 8ttf coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Quinolone resistance protein NorA and IG-heavy chain and IG-light kappa chain
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Multimeric state: hetero trimer
Accessible surface area: 27171.05 Å2
Buried surface area: 4241.02 Å2
Dissociation area: 938.02 Å2
Dissociation energy (ΔGdiss): 3.27 kcal/mol
Dissociation entropy (TΔSdiss): 11.9 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-246915

Macromolecules

Quinolone resistance protein NorA
Chain: A
Length: 424 amino acids
Theoretical weight: 46.65 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A0J7 (Residues: 1-388; Coverage: 100%)
Gene name: norA
Pfam: Major Facilitator Superfamily
Quinolone resistance protein NorA in PDB entry 8ttf, assembly 1, front view.
Quinolone resistance protein NorA in PDB entry 8ttf, assembly 1, side view.
Quinolone resistance protein NorA in PDB entry 8ttf, assembly 1, top view.
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Heavy Chain of FabDA1 Variable Domain
Chain: C
Length: 128 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Heavy Chain of FabDA1 Variable Domain in PDB entry 8ttf, assembly 1, front view.
Heavy Chain of FabDA1 Variable Domain in PDB entry 8ttf, assembly 1, side view.
Heavy Chain of FabDA1 Variable Domain in PDB entry 8ttf, assembly 1, top view.
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Light Chain of FabDA1 Variable Domain
Chain: D
Length: 105 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Light Chain of FabDA1 Variable Domain in PDB entry 8ttf, assembly 1, front view.
Light Chain of FabDA1 Variable Domain in PDB entry 8ttf, assembly 1, side view.
Light Chain of FabDA1 Variable Domain in PDB entry 8ttf, assembly 1, top view.
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