› 8u1h

Electron Microscopy
3Å resolution

Axle-less Bacillus sp. PS3 F1 ATPase mutant

Released:
DOI: 10.2210/pdb8u1h/pdb
PDB entry 8u1h coloured by chain, front view.
PDB entry 8u1h coloured by chain, side view.
PDB entry 8u1h coloured by chain, top view.
Source organism: Bacillus sp. PS3
Primary publication:
The molecular structure of an axle-less F1-ATPase.
Furlong EJ, Reininger-Chatzigiannakis IP, Zeng YC, Brown SHJ, Sobti M, Stewart AG
Biochim Biophys Acta Bioenerg 149521 (2024)
PMID: 39428050
Related structures: EMD-41811

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281477 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 502 amino acids
Theoretical weight: 54.91 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P09219 (Residues: 1-502; Coverage: 100%)
Gene name: atpA
Sequence domains:
ATP synthase subunit beta Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 484 amino acids
Theoretical weight: 53.42 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P07677 (Residues: 1-473; Coverage: 100%)
Gene name: atpD
Sequence domains:
ATP synthase gamma chain Chain: G

Ligands and Environments

4 bound ligands:
Ligand ANP 4 x ANP
Ligand MG 4 x MG
Ligand ADP 1 x ADP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-41811
Expression system: Escherichia coli

Quick links

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 8u1h found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-12183
Datasets: 1
Total size: 887.9Gb
EMPIAR thumbnail