› 8u1h

Electron Microscopy
3Å resolution

Axle-less Bacillus sp. PS3 F1 ATPase mutant

Released:
DOI: 10.2210/pdb8u1h/pdb
PDB entry 8u1h coloured by chain, front view.
PDB entry 8u1h coloured by chain, side view.
PDB entry 8u1h coloured by chain, top view.
Source organism: Bacillus sp. PS3
Primary publication:
The molecular structure of an axle-less F1-ATPase.
Furlong EJ, Reininger-Chatzigiannakis IP, Zeng YC, Brown SHJ, Sobti M, Stewart AG
Biochim Biophys Acta Bioenerg 149521 (2024)
PMID: 39428050
Related structures: EMD-41811

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-268701 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 502 amino acids
Theoretical weight: 54.91 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3VGF9 (Residues: 1-502; Coverage: 100%)
Gene names: atpA, uncA
Sequence domains:
ATP synthase subunit beta Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 484 amino acids
Theoretical weight: 53.42 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M4U1P9 (Residues: 1-473; Coverage: 100%)
Gene names: atpD, uncD
Sequence domains:
ATP synthase gamma chain Chain: G
Molecule details ›
Chain: G
Length: 263 amino acids
Theoretical weight: 29.68 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M4TPJ7 (Residues: 6-197, 198-260; Coverage: 90%)
Gene names: atpG, uncG
Sequence domains: ATP synthase

Ligands and Environments

4 bound ligands:
Ligand ANP 4 x ANP
Ligand MG 4 x MG
Ligand ADP 1 x ADP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-41811
Expression system: Escherichia coli

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