› 8u2u

X-ray diffraction
1.97Å resolution

Crystal Structure of Acetyl-coenzyme A synthetase from Leishmania infantum (CoA, AMP and potassium bound)

Released:
DOI: 10.2210/pdb8u2u/pdb
PDB entry 8u2u coloured by chain, front view.
PDB entry 8u2u coloured by chain, side view.
PDB entry 8u2u coloured by chain, top view.
Source organism: Leishmania infantum
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-coenzyme A synthetase Chain: A
Molecule details ›
Chain: A
Length: 713 amino acids
Theoretical weight: 79.38 KDa
Source organism: Leishmania infantum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4I093 (Residues: 1-705; Coverage: 100%)
Gene name: LINJ_23_0580
Sequence domains:

Ligands and Environments


Cofactor: Ligand COA 1 x COA
4 bound ligands:
Ligand K 1 x K
Ligand CL 2 x CL
Ligand GOL 1 x GOL
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 58.752Å b: 69.465Å c: 149.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.216
Expression system: Escherichia coli BL21(DE3)

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