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Proteasome 20S Core Particle from Beta 3 D205 deletion

Electron Microscopy
2.16Å resolution
PDB entry 8u7u coloured by chain, front view.
PDB entry 8u7u coloured by chain, side view.
PDB entry 8u7u coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Assembly composition:
hetero 28-mer (preferred)
Entry contents: 14 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Proteasome complex
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Multimeric state: hetero 28-mer
Accessible surface area: 207233.23 Å2
Buried surface area: 111955.81 Å2
Dissociation area: 6,051.64 Å2
Dissociation energy (ΔGdiss): 37.57 kcal/mol
Dissociation entropy (TΔSdiss): 28.6 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-149135

Macromolecules

Proteasome subunit alpha type-5
Chains: E, S
Length: 260 amino acids
Theoretical weight: 28.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32379 (Residues: 1-260; Coverage: 100%)
Gene names: DOA5, G9155, PUP2, YGR253C
Pfam:
Proteasome subunit alpha type-5 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit alpha type-5 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit alpha type-5 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit alpha type-2
Chains: B, P
Length: 250 amino acids
Theoretical weight: 27.19 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23639 (Residues: 1-250; Coverage: 100%)
Gene names: PRE8, PRS4, YML092C
Pfam:
Proteasome subunit alpha type-2 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit alpha type-2 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit alpha type-2 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-1
Chains: H, V
Length: 215 amino acids
Theoretical weight: 23.57 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38624 (Residues: 1-215; Coverage: 100%)
Gene names: J1407, PRE3, YJL001W
Pfam: Proteasome subunit
Proteasome subunit beta type-1 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-1 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-1 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit alpha type-6
Chains: F, T
Length: 234 amino acids
Theoretical weight: 25.63 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40302 (Residues: 1-234; Coverage: 100%)
Gene names: PRE5, YM9924.06, YMR314W
Pfam:
Proteasome subunit alpha type-6 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit alpha type-6 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit alpha type-6 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-6
Chains: 1, M
Length: 241 amino acids
Theoretical weight: 26.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23724 (Residues: 1-241; Coverage: 100%)
Gene names: PRE7, PRS3, PTS1, YBL0407, YBL041W
Pfam: Proteasome subunit
Proteasome subunit beta type-6 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-6 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-6 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-4
Chains: K, Y
Length: 198 amino acids
Theoretical weight: 22.55 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22141 (Residues: 1-198; Coverage: 100%)
Gene names: PRE1, YER012W
Pfam: Proteasome subunit
Proteasome subunit beta type-4 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-4 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-4 in PDB entry 8u7u, assembly 1, top view.
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Probable proteasome subunit alpha type-7
Chains: G, U
Length: 288 amino acids
Theoretical weight: 31.58 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P21242 (Residues: 1-288; Coverage: 100%)
Gene names: O6650, PRC1, PRE10, PRS1, YOR362C
Pfam:
Probable proteasome subunit alpha type-7 in PDB entry 8u7u, assembly 1, front view.
Probable proteasome subunit alpha type-7 in PDB entry 8u7u, assembly 1, side view.
Probable proteasome subunit alpha type-7 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-7
Chains: 2, N
Length: 266 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30657 (Residues: 1-266; Coverage: 100%)
Gene names: PRE4, YFR050C
Pfam: Proteasome subunit
Proteasome subunit beta type-7 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-7 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-7 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-2
Chains: I, W
Length: 261 amino acids
Theoretical weight: 28.3 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25043 (Residues: 1-261; Coverage: 100%)
Gene names: PUP1, YOR157C
Pfam:
Proteasome subunit beta type-2 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-2 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-2 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-5
Chains: L, Z
Length: 287 amino acids
Theoretical weight: 31.67 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30656 (Residues: 1-287; Coverage: 100%)
Gene names: DOA3, P8283.10, PRE2, PRG1, YPR103W
Pfam: Proteasome subunit
Proteasome subunit beta type-5 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-5 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-5 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit alpha type-3
Chains: C, Q
Length: 258 amino acids
Theoretical weight: 28.75 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23638 (Residues: 1-258; Coverage: 100%)
Gene names: PRE9, PRS5, YGR135W
Pfam:
Proteasome subunit alpha type-3 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit alpha type-3 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit alpha type-3 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit alpha type-1
Chains: A, O
Length: 252 amino acids
Theoretical weight: 28.03 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P21243 (Residues: 1-252; Coverage: 100%)
Gene names: PRC2, PRS2, SCL1, YGL011C
Pfam:
Proteasome subunit alpha type-1 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit alpha type-1 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit alpha type-1 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit beta type-3
Chains: J, X
Length: 204 amino acids
Theoretical weight: 22.51 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25451 (Residues: 1-204; Coverage: 100%)
Gene names: PUP3, YER094C
Pfam: Proteasome subunit
Proteasome subunit beta type-3 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit beta type-3 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit beta type-3 in PDB entry 8u7u, assembly 1, top view.
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Proteasome subunit alpha type-4
Chains: D, R
Length: 254 amino acids
Theoretical weight: 28.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40303 (Residues: 1-254; Coverage: 100%)
Gene names: PRE6, YOL038W
Pfam:
Proteasome subunit alpha type-4 in PDB entry 8u7u, assembly 1, front view.
Proteasome subunit alpha type-4 in PDB entry 8u7u, assembly 1, side view.
Proteasome subunit alpha type-4 in PDB entry 8u7u, assembly 1, top view.
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