Structure analysis

High resolution in-situ structure of typeA supercomplex in respiratory chain (I1III2IV1,composite)

Electron Microscopy
2.1Å resolution
Source organism: Sus scrofa
Assembly composition:
hetero 81-mer (preferred)
Entry contents: 68 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 81-mer
PDBe Complex ID: PDB-CPX-245924

Macromolecules

Chain: 1A
Length: 115 amino acids
Theoretical weight: 13.03 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79880 (Residues: 1-115; Coverage: 100%)
Gene names: MT-ND3, MTND3, NADH3, ND3
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3

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Chain: 1B
Length: 258 amino acids
Theoretical weight: 28.5 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VVS8 (Residues: 1-258; Coverage: 100%)
Gene name: NDUFS7
Pfam: NADH ubiquinone oxidoreductase, 20 Kd subunit

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Chain: 1C
Length: 264 amino acids
Theoretical weight: 30.15 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZNN4 (Residues: 1-264; Coverage: 100%)
Gene name: NDUFS3
Pfam: Respiratory-chain NADH dehydrogenase, 30 Kd subunit

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Chain: 1D
Length: 476 amino acids
Theoretical weight: 54.17 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0QM68 (Residues: 1-476; Coverage: 100%)
Gene name: NDUFS2
Pfam: Respiratory-chain NADH dehydrogenase, 49 Kd subunit

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Chain: 1E
Length: 249 amino acids
Theoretical weight: 27.44 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VRV3 (Residues: 1-249; Coverage: 100%)
Gene name: NDUFV2
Pfam: Thioredoxin-like [2Fe-2S] ferredoxin

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Chain: 1F
Length: 464 amino acids
Theoretical weight: 50.64 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1SZP7 (Residues: 1-464; Coverage: 100%)
Gene name: NDUFV1
Pfam:

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Chain: 1H
Length: 318 amino acids
Theoretical weight: 35.62 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A0U1RS44 (Residues: 1-318; Coverage: 100%)
Gene names: MT-ND1, ND1
Pfam: NADH dehydrogenase

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Chain: 1I
Length: 239 amino acids
Theoretical weight: 27.14 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZUN9 (Residues: 1-239; Coverage: 100%)
Gene name: NDUFS8
Pfam: 4Fe-4S dicluster domain

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Chain: 1J
Length: 175 amino acids
Theoretical weight: 19.02 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79882 (Residues: 1-175; Coverage: 100%)
Gene names: MT-ND6, MTND6, NADH6, ND6
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase chain 6

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Chain: 1K
Length: 98 amino acids
Theoretical weight: 10.86 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P56632 (Residues: 1-98; Coverage: 100%)
Gene names: MT-ND4L, MTND4L, NADH4L, ND4L
Pfam: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L

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Chain: 1L
Length: 606 amino acids
Theoretical weight: 68.72 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q9TDR1 (Residues: 1-606; Coverage: 100%)
Gene names: MT-ND5, MTND5, NADH5, ND5
Pfam:

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Chain: 1M
Length: 459 amino acids
Theoretical weight: 51.89 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79881 (Residues: 1-459; Coverage: 100%)
Gene names: MT-ND4, MTND4, NADH4, ND4
Pfam:

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Chain: 1N
Length: 347 amino acids
Theoretical weight: 39.11 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79875 (Residues: 1-347; Coverage: 100%)
Gene names: MT-ND2, MTND2, NADH2, ND2
Pfam:

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Chain: 1O
Length: 357 amino acids
Theoretical weight: 40.48 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SIS9 (Residues: 1-357; Coverage: 100%)
Gene name: NDUFA10
Pfam: Deoxynucleoside kinase

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Chain: 1P
Length: 377 amino acids
Theoretical weight: 42.61 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1ULW2 (Residues: 1-377; Coverage: 100%)
Gene name: NDUFA9
Pfam: NAD dependent epimerase/dehydratase family

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Chain: 1Q
Length: 175 amino acids
Theoretical weight: 19.75 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1SN37 (Residues: 1-175; Coverage: 100%)
Gene name: NDUFS4
Pfam: NADH dehydrogenase ubiquinone Fe-S protein 4

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Chain: 1R
Length: 123 amino acids
Theoretical weight: 13.35 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1THU2 (Residues: 1-123; Coverage: 100%)
Gene name: NDUFS6
Pfam: Zinc-finger domain

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Chain: 1S
Length: 99 amino acids
Theoretical weight: 11.1 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1FN21 (Residues: 1-99; Coverage: 100%)
Gene name: NDUFA2
Pfam: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain

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Chains: 1T, 1U
Length: 156 amino acids
Theoretical weight: 17.34 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1KH26 (Residues: 1-156; Coverage: 100%)
Gene name: NDUFAB1
Pfam: Phosphopantetheine attachment site

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Chain: 1V
Length: 116 amino acids
Theoretical weight: 13.32 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0UWW0 (Residues: 1-116; Coverage: 99%)
Pfam: ETC complex I subunit conserved region

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Chain: 1W
Length: 128 amino acids
Theoretical weight: 14.95 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480VKQ8 (Residues: 1-128; Coverage: 100%)
Pfam: Complex 1 protein (LYR family)

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Chain: 1X
Length: 172 amino acids
Theoretical weight: 20.06 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0K6B5 (Residues: 77-248; Coverage: 69%)
Pfam: CHCH domain

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Chain: 1Y
Length: 141 amino acids
Theoretical weight: 14.69 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LGM4 (Residues: 1-141; Coverage: 100%)
Gene name: NDUFA11
Pfam: Tim17/Tim22/Tim23/Pmp24 family

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Chain: 1Z
Length: 144 amino acids
Theoretical weight: 16.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0K655 (Residues: 1-144; Coverage: 100%)
Gene name: NDUFA13
Pfam: GRIM-19 protein

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Chain: 1a
Length: 70 amino acids
Theoretical weight: 8.03 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0TJQ4 (Residues: 92-161; Coverage: 44%)
Pfam: NADH-ubiquinone oxidoreductase MWFE subunit

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Chain: 1b
Length: 84 amino acids
Theoretical weight: 9.23 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VQ42 (Residues: 1-84; Coverage: 100%)
Gene name: NDUFA3
Pfam: NADH dehydrogenase 1 alpha subcomplex subunit 3

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Chain: 1c
Length: 76 amino acids
Theoretical weight: 8.66 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1EYQ2 (Residues: 77-152; Coverage: 50%)
Pfam: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

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Chain: 1d
Length: 122 amino acids
Theoretical weight: 14.33 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480JRW3 (Residues: 1-122; Coverage: 100%)
Gene name: NDUFC2
Pfam: NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2)

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Chain: 1e
Length: 106 amino acids
Theoretical weight: 12.51 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VX77 (Residues: 1-106; Coverage: 100%)
Gene name: NDUFS5
Pfam: NADH:ubiquinone oxidoreductase, NDUFS5-15kDa

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Chain: 1f
Length: 135 amino acids
Theoretical weight: 15.37 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1IZ33 (Residues: 1-106; Coverage: 100%)
Pfam: MNLL subunit

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Chain: 1g
Length: 154 amino acids
Theoretical weight: 17.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1EYI7 (Residues: 1-154; Coverage: 100%)
Pfam: ESSS subunit of NADH:ubiquinone oxidoreductase (complex I)

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Chain: 1h
Length: 189 amino acids
Theoretical weight: 21.59 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SGC6 (Residues: 1-189; Coverage: 100%)
Gene name: NDUFB5
Pfam: NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit

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Chain: 1i
Length: 128 amino acids
Theoretical weight: 15.65 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1UIV8 (Residues: 1-128; Coverage: 100%)
Gene name: NDUFB6
Pfam: NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit

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Chain: 1j
Length: 105 amino acids
Theoretical weight: 11.94 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1G9F4 (Residues: 1-105; Coverage: 100%)
Gene name: NDUFB2
Pfam: NADH dehydrogenase 1 beta subcomplex subunit 2

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Chain: 1k
Length: 98 amino acids
Theoretical weight: 11.13 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VD84 (Residues: 1-98; Coverage: 100%)
Gene name: NDUFB3
Pfam: NADH-ubiquinone oxidoreductase B12 subunit family

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Chain: 1l
Length: 186 amino acids
Theoretical weight: 21.66 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: B8Y651 (Residues: 1-186; Coverage: 100%)
Gene name: NDUFB8
Pfam: NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8)

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Chain: 1m
Length: 129 amino acids
Theoretical weight: 15.12 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1T6M0 (Residues: 1-129; Coverage: 100%)
Gene name: NDUFB4
Pfam: NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)

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Chain: 1n
Length: 179 amino acids
Theoretical weight: 21.86 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: K7GSE5 (Residues: 43-221; Coverage: 81%)
Gene name: NDUFB9
Pfam: Complex 1 protein (LYR family)

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Chain: 1o
Length: 137 amino acids
Theoretical weight: 16.57 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SCH1 (Residues: 2-137; Coverage: 99%)
Gene name: NDUFB7
Pfam: NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7)

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Chain: 1p
Length: 176 amino acids
Theoretical weight: 20.94 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0R7A9 (Residues: 124-299; Coverage: 59%)
Pfam: NADH-ubiquinone oxidoreductase subunit 10

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Chain: 1q
Length: 145 amino acids
Theoretical weight: 17.16 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SQP4 (Residues: 1-145; Coverage: 100%)
Gene name: NDUFA12
Pfam: NADH ubiquinone oxidoreductase subunit NDUFA12

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Chain: 1r
Length: 113 amino acids
Theoretical weight: 12.65 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8W4F7N8 (Residues: 1-113; Coverage: 100%)
Gene name: NDUFA7
Pfam: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)

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Chain: 1s
Length: 471 amino acids
Theoretical weight: 48.22 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TE42 (Residues: 1-471; Coverage: 100%)
Gene name: NDUFV3
Pfam: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

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Chains: 3A, 3N
Length: 480 amino acids
Theoretical weight: 52.76 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0PK14 (Residues: 1-480; Coverage: 100%)
Gene name: LOC100156879
Pfam:

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Chains: 3B, 3O
Length: 453 amino acids
Theoretical weight: 48.26 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1RPD2 (Residues: 1-453; Coverage: 100%)
Gene names: LOC100524613, UQCRC2
Pfam:

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Chains: 3C, 3P
Length: 379 amino acids
Theoretical weight: 42.84 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q1HBG9 (Residues: 1-379; Coverage: 100%)
Gene names: CYTB, CytB, MT-CYB, cytb
Pfam:

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Chains: 3D, 3Q
Length: 325 amino acids
Theoretical weight: 35.36 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1UNI7 (Residues: 1-325; Coverage: 100%)
Gene name: LOC100525869
Pfam: Cytochrome C1 family

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Chains: 3E, 3I, 3R, 3V
Length: 274 amino acids
Theoretical weight: 29.49 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A480EHC1 (Residues: 1-274; Coverage: 100%)
Pfam:

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Chains: 3F, 3S
Length: 111 amino acids
Theoretical weight: 13.59 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZPR8 (Residues: 1-111; Coverage: 100%)
Gene name: UQCRB
Pfam: Ubiquinol-cytochrome C reductase complex 14kD subunit

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Chains: 3G, 3T
Length: 82 amino acids
Theoretical weight: 9.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D0SHY6 (Residues: 1-82; Coverage: 100%)
Gene name: LOC100524308
Pfam: UcrQ family

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Chains: 3H, 3U
Length: 91 amino acids
Theoretical weight: 10.69 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1JI21 (Residues: 1-91; Coverage: 100%)
Gene name: LOC100524873
Pfam: Ubiquinol-cytochrome C reductase hinge protein

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Chains: 3J, 3W
Length: 64 amino acids
Theoretical weight: 7.41 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1J3N6 (Residues: 1-64; Coverage: 100%)
Gene name: UQCR10
Pfam: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like

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Chains: 3X, 3Y
Length: 56 amino acids
Theoretical weight: 6.56 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SDI2 (Residues: 1-56; Coverage: 100%)
Gene name: UQCR11
Pfam: Ubiquinol-cytochrome C reductase complex, 6.4kD protein

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Chain: 4A
Length: 514 amino acids
Theoretical weight: 56.99 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: O79876 (Residues: 1-514; Coverage: 100%)
Gene names: COI, COXI, MT-CO1, MTCO1
Pfam: Cytochrome C and Quinol oxidase polypeptide I

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Chain: 4B
Length: 227 amino acids
Theoretical weight: 26.13 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P50667 (Residues: 1-227; Coverage: 100%)
Gene names: COII, COX2, COXII, MT-CO2, MTCO2
Pfam:

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Chain: 4C
Length: 261 amino acids
Theoretical weight: 29.75 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q35916 (Residues: 1-261; Coverage: 100%)
Gene names: COIII, COXIII, MT-CO3, MTCO3
Pfam: Cytochrome c oxidase subunit III

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Chain: 4D
Length: 169 amino acids
Theoretical weight: 19.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1T8J9 (Residues: 1-169; Coverage: 100%)
Gene name: LOC100624067
Pfam: Cytochrome c oxidase subunit IV

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Chain: 4E
Length: 152 amino acids
Theoretical weight: 16.77 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SJ34 (Residues: 1-152; Coverage: 100%)
Gene name: COX5A
Pfam: Cytochrome c oxidase subunit Va

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Chain: 4F
Length: 129 amino acids
Theoretical weight: 13.8 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q5S3G4 (Residues: 1-129; Coverage: 100%)
Gene name: COX5B
Pfam: Cytochrome c oxidase subunit Vb

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Chain: 4G
Length: 97 amino acids
Theoretical weight: 10.88 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AEB7 (Residues: 1-97; Coverage: 100%)
Gene names: COX6A2, LOC100626250
Pfam: Cytochrome c oxidase subunit VIa

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Chain: 4H
Length: 86 amino acids
Theoretical weight: 10.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A1XQT1 (Residues: 1-86; Coverage: 100%)
Gene name: COX6B
Pfam: Cytochrome oxidase c subunit VIb

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Chain: 4I
Length: 75 amino acids
Theoretical weight: 8.62 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TXH1 (Residues: 1-75; Coverage: 100%)
Gene name: COX6C
Pfam: Cytochrome c oxidase subunit VIc

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Chain: 4J
Length: 80 amino acids
Theoretical weight: 9.03 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q8SPJ9 (Residues: 1-80; Coverage: 100%)
Gene name: COX7A1
Pfam: Cytochrome c oxidase subunit VII

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Chain: 4K
Length: 80 amino acids
Theoretical weight: 9.17 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BJ57 (Residues: 1-80; Coverage: 100%)
Gene name: COX7B
Pfam: Cytochrome C oxidase chain VIIB

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Chain: 4L
Length: 63 amino acids
Theoretical weight: 7.36 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q1W0Y2 (Residues: 1-63; Coverage: 100%)
Gene names: COX7C, COX7C1
Pfam: Cytochrome c oxidase subunit VIIc

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Chain: 4M
Length: 70 amino acids
Theoretical weight: 7.62 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A1XQT4 (Residues: 1-70; Coverage: 100%)
Gene name: COX8H
Pfam: Cytochrome oxidase c subunit VIII

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Chain: 4N
Length: 82 amino acids
Theoretical weight: 9.32 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A1XQS3 (Residues: 1-82; Coverage: 100%)
Gene name: NDUFA4
Pfam: NADH-ubiquinone reductase complex 1 MLRQ subunit

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