› 8uky

X-ray diffraction
2.4Å resolution

Crystal structure of BAK in complex with inhibiting antibody 14G6

Released:
DOI: 10.2210/pdb8uky/pdb
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PDB entry 8uky coloured by chain, front view.
PDB entry 8uky coloured by chain, side view.
PDB entry 8uky coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel inhibitory BAK antibody enables assessment of non-activated BAK in cancer cells.
Subas Satish HP, Iyer S, Shi MX, Wong AW, Fischer KC, Wardak AZ, Lio D, Brouwer JM, Uren RT, Czabotar PE, Miller MS, Kluck RM
Cell Death Differ (2024)
PMID: 38582955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280178 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
14G6 Fab light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 213 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
14G6 Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 219 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Bcl-2 homologous antagonist/killer Chains: C, D

Ligands and Environments

7 bound ligands:
Ligand SO4 3 x SO4
Ligand CCN 3 x CCN
Ligand PEG 8 x PEG
Ligand 144 2 x 144
Ligand PGE 3 x PGE
Ligand EDO 2 x EDO
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 71.554Å b: 72.622Å c: 75.282Å
α: 103.01° β: 103.98° γ: 112.15°
R-values:
R R work R free
0.217 0.215 0.252
Expression systems:
  • Homo sapiens
  • Escherichia coli

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